THE MURINE IGG1/IGE CLASS SWITCH PROGRAM

被引：96

作者：

SIEBENKOTTEN, G ^{[1
]}

ESSER, C ^{[1
]}

WABL, M ^{[1
]}

RADBRUCH, A ^{[1
]}

机构：

[1] UNIV CALIF SAN FRANCISCO,DEPT MICROBIOL & IMMUNOL,SAN FRANCISCO,CA 94143

来源：

EUROPEAN JOURNAL OF IMMUNOLOGY | 1992年 / 22卷 / 07期

关键词：

D O I：

10.1002/eji.1830220723

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Immunoglobulin class switching is controlled by cytokines. Thus, interleukin-4 (IL-4) directs class switching to both IgG1 and IgE. Consistent with this are the results reported here on restriction endonuclease analysis of active and inactive alleles of the IgH locus in IgE-producing cells. In cells that were stimulated in vitro by lipopolysaccharide and IL-4 the silent alleles preferentially switched to gamma-1, whereas in cells that were stimulated by antigen in vivo both active and inactive alleles switched to epsilon. Thirty percent of the recombined switch regions (S(mu)/S(epsilon) contain S(gamma)1 sequences, which we interpret as footprints of a previous switch to gamma-1. Since this percentage is a minimum estimate, between 30% and 100% of switching to epsilon must occur sequentially via gamma-1.

引用

页码：1827 / 1834

页数：8

共 41 条

[11]

JARRETT EEE, 1982, PROG ALLERGY, V31, P178

[12]

KATZENBERG DR, 1988, J IMMUNOL, V140, P3219

[13]

KEARNEY JF, 1978, J IMMUNOL, V120, P158

[14]

KEARNEY JF, 1979, J IMMUNOL, V123, P1548

[15]

KEPRON MR, 1989, J IMMUNOL, V143, P334

[16] ON IMMUNOGLOBULIN HEAVY-CHAIN GENE SWITCHING - 2 GAMMA-2B GENES ARE REARRANGED VIA SWITCH SEQUENCES IN MPC-11 CELLS BUT ONLY ONE IS EXPRESSED [J].

LANG, RB ;

STANTON, LW ;

MARCU, KB .

NUCLEIC ACIDS RESEARCH, 1982, 10 (02) :611-630

[17] INTERLEUKIN-4 CAUSES ISOTYPE SWITCHING TO IGE IN T-CELL-STIMULATED CLONAL B-CELL CULTURES [J].

LEBMAN, DA ;

COFFMAN, RL .

JOURNAL OF EXPERIMENTAL MEDICINE, 1988, 168 (03) :853-862

[18]

LEBRUN P, 1987, J IMMUNOL, V139, P1459

[19] SWITCH CIRCULAR DNA FORMED IN CYTOKINE-TREATED MOUSE SPLENOCYTES - EVIDENCE FOR INTRAMOLECULAR DNA DELETION IN IMMUNOGLOBULIN CLASS SWITCHING [J].

MATSUOKA, M ;

YOSHIDA, K ;

MAEDA, T ;

USUDA, S ;

SAKANO, H .

CELL, 1990, 62 (01) :135-142

[20] A SIMPLE SALTING OUT PROCEDURE FOR EXTRACTING DNA FROM HUMAN NUCLEATED CELLS [J].

MILLER, SA ;

DYKES, DD ;

POLESKY, HF .

NUCLEIC ACIDS RESEARCH, 1988, 16 (03) :1215-1215

← 1 2 3 4 5 →