MAJOR DNA-POLYMERASE IN CULTURED PLANT-CELLS - PARTIAL-PURIFICATION AND CORRELATION WITH CELL MULTIPLICATION

被引：31

作者：

AMILENI, A ^{[1
]}

SALA, F ^{[1
]}

CELLA, R ^{[1
]}

SPADARI, S ^{[1
]}

机构：

[1] CNR,GENET BIOCHIM EVOLUZ LAB,PAVIA,ITALY

来源：

PLANTA | 1979年 / 146卷 / 05期

关键词：

DNA polymerase; Plant cell suspension culture;

D O I：

10.1007/BF00388827

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A DNA polymerase activity was isolated from cells of Oryza sativa L. grown in suspension culture. Molecular mass (∼ 180,000), optimal requirements for pH (neutral), Mg2+ (5-10 mM), Mn2+ (1 mM), template preference (activated DNA), lack of activity with native or denatured DNA, and sensitivity to N-ethylmaleimide and ionic strength are similar to those of the vertebrate α-polymerase. Like DNA polymerase α, the DNA polymerase described in this work is the most abundant in proliferating cells of Oryza sativa L., Parthenocissus tricuspidata (Siebold et Zucc.) Planchon, Acer pseudoplatanus L., and Medicago sativa L. and responds to changes in the rate of cell multiplication. We therefore postulate that this α-like DNA polymerase is the replicating enzyme of plant cells. © 1979 Springer-Verlag.

引用

页码：521 / 527

页数：7

共 30 条

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