ISOLATION AND CHARACTERIZATION OF THERMOSTABLE CHITINASES FROM BACILLUS-LICHENIFORMIS X-7U

被引：84

作者：

TAKAYANAGI, T ^{[1
]}

AJISAKA, K ^{[1
]}

TAKIGUCHI, Y ^{[1
]}

SHIMAHARA, K ^{[1
]}

机构：

[1] SEIKEI UNIV, FAC ENGN, MUSASHINO, TOKYO 180, JAPAN

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1991年 / 1078卷 / 03期

关键词：

CHITINASE; THERMOSTABLE; (BACILLUS);

D O I：

10.1016/0167-4838(91)90163-T

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Four kinds of thermostable chitinase were isolated from the cell-free culture broth of Bacillus licheniformis X-7u by successive column chromatographies on Butyl-Toyopearl, Q-Sepharose, and Sephacryl S-200. We named the enzymes chitinases I(89 kDa), II(76 kDa), III(66 kDa) and IV(59 kDa). Chitinases II, III and IV possessed extremely high optimum temperatures (70-80-degrees-C), showing remarkable heat stability. Chitinases II, Ill and IV produced (GlcNAc)2 and GlcNAc from colloidal chitin and chitinase I predominantly produced (GlcNAc)2. The action pattern of chitinase I on PN-(GlcNAc)4 also showed a stronger propensity to cleave off the (GlcNAc)2 unit from the non-reducing end than the other three chitinases. Chitinases II, III and IV catalyzed a transglycosylation reaction that converted (GlcNAc)4 into (GlcNAc)6.

引用

页码：404 / 410

页数：7

共 24 条

[21] ISOLATION AND IDENTIFICATION OF A THERMOPHILIC BACTERIUM PRODUCING N,N'-DIACETYLCHITOBIOSE FROM CHITIN [J].