RESOLUTION OF LIPOLYSIS MIXTURES FROM SOYBEAN OIL BY A SOLID-PHASE EXTRACTION PROCEDURE

被引：22

作者：

NEFF, WE

ZEITOUN, MAM

WEISLEDER, D

机构：

[1] UNIV ALEXANDRIA,FAC AGR SABA BASHA,DEPT FOOD SCI,ALEXANDRIA,EGYPT

[2] USDA ARS,NATL CTR AGR UTILIZAT RES,PEORIA,IL 61604

来源：

JOURNAL OF CHROMATOGRAPHY | 1992年 / 589卷 / 1-2期

关键词：

D O I：

10.1016/0021-9673(92)80045-V

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A solid-phase extraction procedure was developed to rapidly resolve the lipolysis mixtures of free fatty acids, mono- and diacylglycerols from lipase (E.C. 3.1.1.3) cleavage of soybean oil triacylglycerols. Accuracy of the lipolysis procedure against standard mixtures of triacylglycerols showed coefficients of variance of 5% between known and observed fatty acid composition at glycerol carbon 2. An average coefficient of variance of 5% was obtained between results of the lipolysis procedure, fatty acid composition by gas chromatography, and the results by C-13 nuclear magnetic resonance stereospecific analysis for fatty acid composition at glycerol carbons 1,3 and 2 of intact triacylglycerol mixtures.

引用

页码：353 / 357

页数：5

共 28 条

[21] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR QUANTITATION OF FREE ACIDS, MONOGLYCERIDES, DIGLYCERIDES AND TRIGLYCERIDES USING AN INFRARED DETECTOR [J].