T-DNA AS A GENE TAG

被引：42

作者：

WALDEN, R

HAYASHI, H

SCHELL, J

机构：

[1] Max-Planck-Institut Für Züchtungsforschung, Kö Ln, D5000

来源：

PLANT JOURNAL | 1991年 / 1卷 / 03期

关键词：

D O I：

10.1046/j.1365-313X.1991.t01-6-00999.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The characteristics of Agrobacterium-mediated plant transformation are such that the T-DNA can be used as an insertional mutagen and, by extension, a gene tag. An increasing number of mutants have been obtained as a result of T-DNA insertion and the versatility of this experimental system can be exploited further to tag sequences of DNA which control gene expression. While the isolation of specific mutations by T-DNA tagging in the past has been fortuitous, T-DNA-based vectors have the potential to generate transgenic plants with specific, selectable mutations.

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页码：281 / 288

页数：8

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