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IMMUNOCHEMISTRY AND LOCALIZATION OF THE ENZYME DISAGGREGATASE IN METHANOSARCINA-MAZEI

被引：10
作者：
DEMACARIO, EC
MACARIO, AJL
MOK, T
BEVERIDGE, TJ
机构：
[1] SUNY Albany, SCH PUBL HLTH, DEPT BIOMED SCI, ALBANY, NY 12222 USA
[2] UNIV GUELPH, DEPT MICROBIOL, GUELPH N1G 2W1, ONTARIO, CANADA
[3] UNIV GUELPH, COLL BIOL SCI, CANADIAN BACTERIAL DIS NETWORK, GUELPH N1G 2W1, ONTARIO, CANADA
来源：
JOURNAL OF BACTERIOLOGY | 1993年 / 175卷 / 10期
关键词：
D O I：
10.1128/JB.175.10.3115-3120.1993
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The enzyme disaggregatase (Dag) from Methanosarcina mazei was studied immunochemically. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of purified Dag under reducing and nonreducing conditions revealed a single band with a 94-kDa molecular mass. Dag was found to be immunogenic in rabbits; a polyclonal antibody probe was prepared and used to detect the enzyme by slide immunoenzymatic assay, immunofluorescence, and immunoblotting in various species of Methanosarcina known to convert from packets to single cells, including M. mazei. The enzyme could not be detected in other members of the family Methanosarcinaceae that do not convert. By immunogold electron microscopy, Dag was mapped to the cell wall of packets and to the cell membrane of single cells of two M. mazei strains.
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收藏
页码：3115 / 3120
页数：6
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