FRACTIONATION AND PURIFICATION OF THE THIOL PROTEINASES FROM PAPAYA LATEX

被引：10

作者：

DEKEYSER, PM

DESMEDT, S

DEMEESTER, J

LAUWERS, A

机构：

[1] Laboratories for General Biochemistry and Physical Pharmacy, Department of Pharmaceutics, University of Ghent, 9000 Ghent

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1994年 / 656卷 / 01期

关键词：

D O I：

10.1016/0378-4347(94)00083-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Three cysteine proteinases, i.e. chymopapain, papaya proteinase IV and proteinase III, were purified to homogeneity from papaya latex using a combination of ion-exchange chromatography and hydrophobic interaction chromatography. During the purification procedure, the thiol-groups of the active center were reversibly blocked as mixed disulfides with 2-thiopyridone. Homogeneity was proved electrophoretically by native polyacrylamide gel electrophoresis (PAGE), sodium dodecyl sulfate (SDS)-PAGE and rechromatography on a Mono S 5/5 column at pH 5.0.

引用

页码：203 / 208

页数：6

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