INFLUENCE OF UNSATURATED FATTY-ACIDS, MEMBRANE FLUIDITY AND OXYGENATION ON THE SURVIVAL OF AN ESCHERICHIA-COLI FATTY-ACID AUXOTROPH FOLLOWING GAMMA-IRRADIATION

被引：42

作者：

YATVIN, MB

GIPP, JJ

DENNIS, WH

机构：

[1] UNIV WISCONSIN,SCH MED,DEPT HUMAN ONCOL,MADISON,WI 53706

[2] UNIV WISCONSIN,SCH MED,DEPT PHYSIOL,MADISON,WI 53706

来源：

INTERNATIONAL JOURNAL OF RADIATION BIOLOGY | 1979年 / 35卷 / 06期

关键词：

D O I：

10.1080/09553007914550651

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Escherichia coli K1060, a fatty acid auxotroph unable to either synthesize or degrade unsaturated fatty acids (uFAs), was used to study the effect of membrane fluidity on survival after exposure to ionizing radiation. Using this strain of E. coli, significant alterations in the fatty acid composition of the membrane have been produced and verified by gas chromatography. Linolenic, oleic, elaidic and palmitelaidic acids were the uFAs used. Survival above the transition temperature (Tt) (liquid crystalgel) was comparable for these fatty-acid-supplemented membranes after exposure to γirradiation, whereas γirradiation below Tt resulted in a significant decrease in survival. An oxygen enhancement effect was observed for each experimental condition employed. © 1979 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

引用

页码：539 / 548

页数：10

共 27 条

[11]

GHOLIPOUR-KHALILI K, 1978, Journal of Science of the Hiroshima University Series B Division 1 (Zoology), V27, P175

[12] CHANGES IN PHOSPHOLIPID COMPOSITION OF ESCHERICHIA-COLI FOLLOWING GAMMA-IRRADIATION AND UV-IRRADIATION [J].

JACOBSON, AF ;

YATVIN, MB .

RADIATION RESEARCH, 1976, 66 (02) :247-266

[13]

LETELLIER L, 1976, J MICROSC BIOL CELL, V25, P191

[14] RAPID PREPARATION OF FATTY ACID ESTERS FROM LIPIDS FOR GAS CHROMATOGRAPHIC ANALYSIS [J].

METCALFE, LD ;

SCHMITZ, AA ;

PELKA, JR .

ANALYTICAL CHEMISTRY, 1966, 38 (03) :514-&

[15] AGING OF NEUROSPORA-CRASSA .4. INDUCTION OF SENESCENCE IN WILD-TYPE BY DIETARY AMINO-ACID ANALOGS AND REVERSAL BY ANTIOXIDANTS AND MEMBRANE STABILIZERS [J].

MUNKRES, KD .

MECHANISMS OF AGEING AND DEVELOPMENT, 1976, 5 (03) :171-191

[16] CORRELATION OF IN-VIVO AND IN-VITRO PHASE TRANSITIONS OF MEMBRANE LIPIDS IN ESCHERICHIA-COLI [J].

OVERATH, P ;

SCHAIRER, HU ;

STOFFEL, W .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1970, 67 (02) :606-+

[17] RADIOPROTECTIVE EFFECT OF SUPEROXIDE-DISMUTASE ON MODEL PHOSPHOLIPID MEMBRANES [J].

PETKAU, A ;

CHELACK, WS .

BIOCHIMICA ET BIOPHYSICA ACTA, 1976, 433 (03) :445-456

[18] DOSE-RATE AND OXYGEN EFFECTS IN MODELS OF LIPID-MEMBRANES - LINOLEIC-ACID [J].

RALEIGH, JA ;

KREMERS, W ;

GABOURY, B .

INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, 1977, 31 (03) :203-213

[19] ASSOCIATION OF NUCLEUS AND MEMBRANE OF BACTERIA - A MORPHOLOGICAL STUDY [J].

RYTER, A .

BACTERIOLOGICAL REVIEWS, 1968, 32 (01) :39-&

[20] LIPIDS CONTAINING TRANS-UNSATURATED FATTY ACIDS CHANGE TEMPERATURE CHARACTERISTIC OF THIOMETHYLGALACTOSIDE ACCUMULATION IN ESCHERICHIA COLI [J].

SCHAIRER, HU ;

OVERATH, P .

JOURNAL OF MOLECULAR BIOLOGY, 1969, 44 (01) :209-+

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