DIFFERENTIAL INVOLVEMENT OF PROTEIN-KINASE-C IN TRANSMITTER RELEASE AND RESPONSE TO EXCITATORY AMINO-ACIDS IN CULTURED CEREBELLAR NEURONS

被引：27

作者：

EBOLI, ML ^{[1
]}

CIOTTI, MT ^{[1
]}

MERCANTI, D ^{[1
]}

CALISSANO, P ^{[1
]}

机构：

[1] CNR, INST NEUROBIOL, I-00137 ROME, ITALY

来源：

NEUROCHEMICAL RESEARCH | 1993年 / 18卷 / 02期

关键词：

CEREBELLAR GRANULES; EXCITOTOXICITY; RESISTANCE; PROTEIN KINASE-C; TRANSLOCATION; TRANSMITTER RELEASE;

D O I：

10.1007/BF01474675

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cerebellar granule cells cultured in the presence of a differentiating factor isolated from rabbit serum exhibit, at variance with those cultured in fetal calf serum, an almost complete resistance to excitatory aminoacid (EAA)-induced citotoxicity. We investigated the behaviour of protein kinase C (PKC), strongly implicated in EAA cytotoxicity, in the two types of culture. Phorbol esters, used to monitor the enzyme, enhanced the depolarization-evoked release of D-[H-3]aspartate, but less effectively in factor-conditioned cells. EAAs increased phorbol esters binding in both cultures, but the effect was briefly lasting in factor-conditioned cells. The different behaviour of PKC is postulated to be causally related to different response to EAA of the cultures.

引用

页码：133 / 138

页数：6

共 34 条

[1] INTRASYNAPTOSOMAL FREE CALCIUM-CONCENTRATION IS INCREASED BY PHORBOL ESTERS VIA A 1,4-DIHYDROPYRIDINE-SENSITIVE (L-TYPE) CA-2+ CHANNEL [J].