THE SEROLOGY OF HEPATITIS-C VIRUS (HCV) INFECTION - ANTIBODY CROSS-REACTION IN THE HYPERVARIABLE REGION-1

被引：12

作者：

CARDOSO, MD

SIEMONEIT, K

NEMECEK, V

EPPLE, S

KOERNER, K

KUBANEK, B

机构：

[1] UNIV ULM,DEPT TRANSFUS MED,D-89081 ULM,GERMANY

[2] UNIV ULM,GERMAN RED CROSS,D-89081 ULM,GERMANY

[3] NATL PUBL HLTH INST,PRAGUE,CZECH REPUBLIC

来源：

ARCHIVES OF VIROLOGY | 1995年 / 140卷 / 10期

关键词：

D O I：

10.1007/BF01384335

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We determined the NS1/E2 N-terminal sequence including the hypervariable region 1 (HVR1) from five individuals chronically infected with HCV: two from the Czech Republic and three from Germany. From each sequence, six 12-mer overlapping peptides were synthesized and used in a peptide scan to evaluate seroreactivity of each of those patients, as well as three anti-HCV positive blood donors to the different isolates. We could show the general presence of antibodies to multiple HVR1 specific sequences reflecting the existence of multiple variants in infected persons. Finally, we observed the persistance of HCV infections in all individuals despite an active humoral response directed against the virus.

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页码：1705 / 1713

页数：9

相关论文

共 16 条

[1] GENOMIC CHARACTERIZATION AND MUTATION-RATE OF HEPATITIS-C VIRUS ISOLATED FROM A PATIENT WHO CONTRACTED HEPATITIS DURING AN EPIDEMIC OF NON-A, NON-B HEPATITIS IN JAPAN [J].

ABE, K ;

INCHAUSPE, G ;

FUJISAWA, K .

JOURNAL OF GENERAL VIROLOGY, 1992, 73 :2725-2729

[2]

BEENHOUWER H, 1992, VOX SANG, V63, P198

[3] PREVALENCE OF HCV-RNA-POSITIVE BLOOD-DONORS AND CORRELATION TO ELISA AND RIBA STATUS [J].

CARDOSO, MD ;

KOERNER, K ;

EPPLE, S ;

KUBANEK, B .

ANNALS OF HEMATOLOGY, 1993, 66 (03) :147-151

[4] GENETIC ORGANIZATION AND DIVERSITY OF THE HEPATITIS-C VIRUS [J].

CHOO, QL ;

RICHMAN, KH ;

HAN, JH ;

BERGER, K ;

LEE, C ;

DONG, C ;

GALLEGOS, C ;

COIT, D ;

MEDINASELBY, A ;

BARR, PJ ;

WEINER, AJ ;

BRADLEY, DW ;

KUO, G ;

HOUGHTON, M .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (06) :2451-2455

[5] HYPERVARIABLE REGIONS IN THE PUTATIVE GLYCOPROTEIN OF HEPATITIS-C VIRUS [J].

HIJIKATA, M ;

KATO, N ;

OOTSUYAMA, Y ;

NAKAGAWA, M ;

OHKOSHI, S ;

SHIMOTOHNO, K .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1991, 175 (01) :220-228

[6] QUANTITATION OF RELATIVE FITNESS AND GREAT ADAPTABILITY OF CLONAL POPULATIONS OF RNA VIRUSES [J].

HOLLAND, JJ ;

DELATORRE, JC ;

CLARKE, DK ;

DUARTE, E .

JOURNAL OF VIROLOGY, 1991, 65 (06) :2960-2967

[7] GENOMIC STRUCTURE OF THE HUMAN PROTOTYPE STRAIN-H OF HEPATITIS-C VIRUS - COMPARISON WITH AMERICAN AND JAPANESE ISOLATES [J].

INCHAUSPE, G ;

ZEBEDEE, S ;

LEE, DH ;

SUGITANI, M ;

NASOFF, M ;

PRINCE, AM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (22) :10292-10296

[8] USE OF CONSERVED SEQUENCES FROM HEPATITIS-C VIRUS FOR THE DETECTION OF VIRAL-RNA IN INFECTED SERA BY POLYMERASE CHAIN-REACTION [J].

INCHAUSPE, G ;

ABE, K ;

ZEBEDEE, S ;

NASOFF, M ;

PRINCE, AM .

HEPATOLOGY, 1991, 14 (04) :595-600

[9] CHARACTERIZATION OF HYPERVARIABLE REGIONS IN THE PUTATIVE ENVELOPE PROTEIN OF HEPATITIS-C VIRUS [J].

KATO, N ;

OOTSUYAMA, Y ;

OHKOSHI, S ;

NAKAZAWA, T ;

SEKIYA, H ;

HIJIKATA, M ;

SHIMOTOHNO, K .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1992, 189 (01) :119-127

[10] HUMORAL IMMUNE-RESPONSE TO HYPERVARIABLE REGION-1 OF THE PUTATIVE ENVELOPE GLYCOPROTEIN (GP70) OF HEPATITIS-C VIRUS [J].

KATO, N ;

SEKIYA, H ;

OOTSUYAMA, Y ;

NAKAZAWA, T ;

HIJIKATA, M ;

OHKOSHI, S ;

SHIMOTOHNO, K .

JOURNAL OF VIROLOGY, 1993, 67 (07) :3923-3930