Competitive inhibition of the 5-lipoxygenase-catalysed linoleate oxidation by arachidonic and 5-hydroperoxy-eicosatetraenoic acids

被引：8

作者：

Mirzoeva, OK ^{[1
]}

Sudina, GF ^{[1
]}

Pushkareva, MA ^{[1
]}

Varfolomeev, SD ^{[1
]}

机构：

[1] MOSCOW MV LOMONOSOV STATE UNIV,AN BELOZERSKY INST PHYSICOCHEM BIOL,MOSCOW 000958,RUSSIA

来源：

FEBS LETTERS | 1995年 / 377卷 / 03期

基金：

俄罗斯基础研究基金会;

关键词：

5-lipoxygenase; linoleic acid; arachidonic acid;

D O I：

10.1016/0014-5793(95)01350-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Linoleic and arachidonic acids are competing substrates for 5-lipoxygenase from barley. When these two substrates are added simultaneously, arachidonic acid acts as a competitive inhibitor of linoleic acid oxidation with K-i of 20 mu M, the same value as the Michaelis constant for arachidonate oxygenation by this enzyme (22 +/- 3 mu M). Linoleic acid hydroperoxide accumulated in the reaction mixture does not inhibit the enzymatic process, while ararhidonic acid hydroperoxy product (5-hydroperoxy-6,8,11,14-eicosatetraenoic acid) inhibits it with very low K-i equal to 0.5 mu M.

引用

页码：306 / 308

页数：3

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