INCREASED THERMAL-STABILITY OF BACILLUS-LICHENIFORMIS ALPHA-AMYLASE IN THE PRESENCE OF VARIOUS ADDITIVES

被引：55

作者：

ASTHER, M ^{[1
]}

MEUNIER, JC ^{[1
]}

机构：

[1] INRA,CTR BIOTECHNOL AGROIND,CHIM BIOL LAB,F-78850 THIVERVAL GRIGNON,FRANCE

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 1990年 / 12卷 / 11期

关键词：

organic solvents; thermostability; thermostabilizing additives; α-Amylase;

D O I：

10.1016/0141-0229(90)90030-T

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The influence of various additives such as polyhydric alcohols, sugars, polyethylene glycol, and substrates on the thermostability of Bacillus licheniformis α-amylase has been studied. Results obtained showed a thermostabilizing effect of most of the additives tested (ethylene glycol, glycerol, sorbitol, glucose, maltose, maltotriose, and polyethylene glycol). The stabilizing efficiency appears to be strongly dependent on additive concentration. α-Amylase thermostability has also been assayed in organic solvents. In these media, particularly in octane, α-amylase acquired a remarkable stability since no deactivation occurred at 75°C for at least 90 min. © 1990.

引用

页码：902 / 905

页数：4

共 30 条

[1] THERMOSTABILITY OF MEMBRANE ENZYMES IN ORGANIC-SOLVENTS
AYALA, G
DEGOMEZPUYOU, MT
GOMEZPUYOU, A
DARSZON, A
[J]. FEBS LETTERS, 1986, 203 (01) : 41 - 43
[2] INCREASED THERMAL-STABILITY OF PROTEINS IN THE PRESENCE OF SUGARS AND POLYOLS
BACK, JF
OAKENFULL, D
SMITH, MB
[J]. BIOCHEMISTRY, 1979, 18 (23) : 5191 - 5196
[3] AMYLASES, ALPHA AND BETA
BERNFELD, P
[J]. METHODS IN ENZYMOLOGY, 1955, 1 : 149 - 158
[4] GLYCEROL AS AN ENZYME-STABILIZING AGENT - EFFECTS ON ALDEHYDE DEHYDROGENASE
BRADBURY, SL
JAKOBY, WB
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1972, 69 (09) : 2373 - &
[5] EFFECT OF ADDITIVES ON THE THERMOSTABILITY OF BACILLUS-STEAROTHERMOPHILUS ALPHA-AMYLASE
BRUMM, PJ
TEAGUE, WM
[J]. BIOTECHNOLOGY LETTERS, 1989, 11 (08) : 541 - 544
[6] PURIFICATION AND CHARACTERIZATION OF A THERMOSTABLE ALPHA-AMYLASE FROM BACILLUS LICHENIFORMIS
CHIANG, JP
ALTER, JE
STERNBERG, M
[J]. STARKE, 1979, 31 (03): : 86 - 92
[7] CITRI N, 1973, ADV ENZYMOL RAMB, V37, P397
[8] EZURE Y, 1988, AGR BIOL CHEM TOKYO, V52, P1073
[9] THE EFFECT OF POLYHYDRIC ALCOHOLS ON THE THERMAL-DENATURATION OF LYSOZYME AS MEASURED BY DIFFERENTIAL SCANNING CALORIMETRY
FUJITA, Y
IWASA, Y
NODA, Y
[J]. BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN, 1982, 55 (06) : 1896 - 1900
[10] EFFECT OF POLYOLS ON FUNGAL ALPHA-AMYLASE THERMOSTABILITY
GRABER, M
COMBES, D
[J]. ENZYME AND MICROBIAL TECHNOLOGY, 1989, 11 (10) : 673 - 677

← 1 2 3 →