A PUTATIVE BETA-GLUCANASE PSEUDOGENE BEHIND THE POTATO GBSS GENE

被引:1
作者
VANDERLEIJ, FR
ABELN, ECA
HESSELINGMEINDERS, A
FEENSTRA, WJ
机构
[1] Department of Genetics, University of Groningen, Haren, NL-9751 NN
关键词
SOLANUM-TUBEROSUM L; AMF LOCUS; WAXY LOCUS; GLUCANASE; GLUCOSIDASE; STARCH SYNTHASE DNA SEQUENCE;
D O I
10.1007/BF00028815
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We identified an open reading frame (ORF) which is located closely behind the gene encoding granule-bound starch synthase (GBSS) of potato (Solanum tuberosum L.). The ORF ends with a perfect 43 bp direct repeat, which carries the stop triplet precisely at the beginning of the second repeat. The deduced protein shows homology with all known isoforms of plant beta-1,3-glucanases and beta-1,3-1,4-glucanases. Although the DNA sequence is unique in potato and tomato (Lycopersicon esculentum L.), no expression of the gene was found in these species. Taken together with the unusual codon usage and length of the predicted protein, this sequence could be a pseudogene.
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页码:567 / 571
页数:5
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