SIMULTANEOUS DETECTION OF THIOLS AND DISULFIDES BY CAPILLARY ELECTROPHORESIS ELECTROCHEMICAL DETECTION USING A MIXED-VALENCE RUTHENIUM CYANIDE-MODIFIED MICROELECTRODE

被引：48

作者：

ZHOU, JX ^{[1
]}

OSHEA, TJ ^{[1
]}

LUNTE, SM ^{[1
]}

机构：

[1] UNIV KANSAS,CTR BIOANALYT RES,LAWRENCE,KS 66047

来源：

JOURNAL OF CHROMATOGRAPHY A | 1994年 / 680卷 / 01期

关键词：

D O I：

10.1016/0021-9673(94)80077-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Thiols and disulfides are separated and detected by capillary electrophoresis-electrochemical detection using a mixed-valence ruthenium cyanide-modified microelectrode. A carbon fiber array microelectrode was employed to maximize the signal-to-noise ratio. Detection limits for glutathione disulfide, cystine and homocystine were 2.5, 1.3 and 1.1 fmol, respectively. The response for cystine was linear over two orders of magnitude with a correlation coefficient of 0.992. The long-term stability and overall reproducibility of the electrode were investigated and found to be highly dependent on the cation concentration in the electrophoretic buffer. The selectivity of this technique for disulfides was demonstrated by the detection of cystine in the urine of a patient with kidney stones.

引用

页码：271 / 277

页数：7

共 28 条

[11] ELECTROCATALYTIC OXIDATION AND DETERMINATION OF ARSENIC(III) ON A GLASSY-CARBON ELECTRODE MODIFIED WITH A THIN-FILM OF MIXED-VALENT RUTHENIUM(III, II) CYANIDE [J].