TALES OF POLY(A) - A REVIEW

被引：149

作者：

MUNROE, D ^{[1
]}

JACOBSON, A ^{[1
]}

机构：

[1] UNIV MASSACHUSETTS, SCH MED, DEPT MOLEC GENET & MICROBIOL, 55 LAKE AVE N, WORCESTER, MA 01655 USA

来源：

GENE | 1990年 / 91卷 / 02期

关键词：

3′-translational enhancer; poly(A)-binding protein; Protein synthesis;

D O I：

10.1016/0378-1119(90)90082-3

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Until recently, evidence to support a translational role for the 3′-poly(A) tract of eukaryotic mRNAs has been mostly indirect, including: a correlation between the adenylation status of individual mRNAs and their translatability in vivo or in vitro, the demonstration that exogenously added poly(A) is a potent competitive inhibitor of the translation of poly(A)+mRNA, but not poly(A)-mRNAs in vitro, and a correlation between the abundance and stability of poly(A)-binding proteins (PABPs) and the rate of translational initiation in vivo. However, more recent studies demonstrate directly that poly(A)+mRNAs can initiate translation more efficiently than poly(A)-mRNAs, and indicate that this effect is: (i) targeted to the formation of 80S initiation complexes, and (ii) likely to be mediated by the cytoplasmic PABP. We suggest that the 3′-poly(A) tail should be considered a translational enhancer which may stimulate translational initiation in much the same way that transcriptional enhancers are thought to stimulate transcriptional initiation. © 1990.

引用

页码：151 / 158

页数：8

共 92 条

[11] VACCINIA VIRUS PRODUCES LATE MESSENGER-RNAS BY DISCONTINUOUS SYNTHESIS [J].