EXPRESSION OF THE ALPHA-THIONIN GENE FROM BARLEY IN TOBACCO CONFERS ENHANCED RESISTANCE TO BACTERIAL PATHOGENS

被引：137

作者：

CARMONA, MJ ^{[1
]}

MOLINA, A ^{[1
]}

FERNANDEZ, JA ^{[1
]}

LOPEZFANDO, JJ ^{[1
]}

GARCIAOLMEDO, F ^{[1
]}

机构：

[1] UPM,ETSI AGRON,E-28040 MADRID,SPAIN

来源：

PLANT JOURNAL | 1993年 / 3卷 / 03期

关键词：

D O I：

10.1046/j.1365-313X.1993.t01-24-00999.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Thionins are cysteine-rich, 5 kDa polypeptides which are toxic to plant pathogens in vitro. Expression of the gene encoding alpha-thionin from barley endosperm, under the 35S promoter f rom cauliflower mosaic virus, conferred to transgenic tobacco enhanced resistance to the bacterial plant pathogens Pseudomonas syringae pv. tabaci 153 and P. syringae pv. syringae. The barley alpha-thionin gene, which has two introns, was correctly spliced in tobacco. The alpha-thionin in transgenic plants had the expected mobility in the gradient, when separated by high-performance liquid chromatography, reacted with monospecific antibodies and showed the expected antibiotic properties in vitro.

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页码：457 / 462

页数：6

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