EXPRESSION OF THE ALPHA-THIONIN GENE FROM BARLEY IN TOBACCO CONFERS ENHANCED RESISTANCE TO BACTERIAL PATHOGENS

被引：137

作者：

CARMONA, MJ ^{[1
]}

MOLINA, A ^{[1
]}

FERNANDEZ, JA ^{[1
]}

LOPEZFANDO, JJ ^{[1
]}

GARCIAOLMEDO, F ^{[1
]}

机构：

[1] UPM,ETSI AGRON,E-28040 MADRID,SPAIN

来源：

PLANT JOURNAL | 1993年 / 3卷 / 03期

关键词：

D O I：

10.1046/j.1365-313X.1993.t01-24-00999.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Thionins are cysteine-rich, 5 kDa polypeptides which are toxic to plant pathogens in vitro. Expression of the gene encoding alpha-thionin from barley endosperm, under the 35S promoter f rom cauliflower mosaic virus, conferred to transgenic tobacco enhanced resistance to the bacterial plant pathogens Pseudomonas syringae pv. tabaci 153 and P. syringae pv. syringae. The barley alpha-thionin gene, which has two introns, was correctly spliced in tobacco. The alpha-thionin in transgenic plants had the expected mobility in the gradient, when separated by high-performance liquid chromatography, reacted with monospecific antibodies and showed the expected antibiotic properties in vitro.

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页码：457 / 462

页数：6

相关论文

共 32 条

[21]

LEAH R, 1991, J BIOL CHEM, V266, P1564

[22] DEFENSINS - ENDOGENOUS ANTIBIOTIC PEPTIDES OF ANIMAL-CELLS [J].

LEHRER, RI ;

GANZ, T ;

SELSTED, ME .

CELL, 1991, 64 (02) :229-230

[23] EXPRESSION OF A BARLEY RIBOSOME-INACTIVATING PROTEIN LEADS TO INCREASED FUNGAL PROTECTION IN TRANSGENIC TOBACCO PLANTS [J].

LOGEMANN, J ;

JACH, G ;

TOMMERUP, H ;

MUNDY, J ;

SCHELL, J .

BIO-TECHNOLOGY, 1992, 10 (03) :305-308

[24] RAPID MICRO SCALE METHOD FOR DETECTION OF LYSOPINE AND NOPALINE DEHYDROGENASE-ACTIVITIES [J].

OTTEN, LABM ;

SCHILPEROORT, RA .

BIOCHIMICA ET BIOPHYSICA ACTA, 1978, 527 (02) :497-500

[25] SYNTHESIS AND PROCESSING OF THIONIN PRECURSORS IN DEVELOPING ENDOSPERM FROM BARLEY (HORDEUM-VULGARE-L) [J].

PONZ, F ;

PAZARES, J ;

HERNANDEZLUCAS, C ;

CARBONERO, P ;

GARCIAOLMEDO, F .

EMBO JOURNAL, 1983, 2 (07) :1035-1040

[26] CLONING AND NUCLEOTIDE-SEQUENCE OF A CDNA-ENCODING THE PRECURSOR OF THE BARLEY TOXIN ALPHA-HORDOTHIONIN [J].

PONZ, F ;

PAZARES, J ;

HERNANDEZLUCAS, C ;

GARCIAOLMEDO, F ;

CARBONERO, P .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1986, 156 (01) :131-135

[27]

REIMANNPHILIPP U, 1989, J BIOL CHEM, V264, P8978

[28] NUCLEOTIDE-SEQUENCE AND ENDOSPERM-SPECIFIC EXPRESSION OF THE STRUCTURAL GENE FOR THE TOXIN ALPHA-HORDOTHIONIN IN BARLEY (HORDEUM-VULGARE-L) [J].

RODRIGUEZPALENZUELA, P ;

PINTORTORO, JA ;

CARBONERO, P ;

GARCIAOLMEDO, F .

GENE, 1988, 70 (02) :271-281

[29]

Sambrook J., 1989, MOL CLONING LAB MANU

[30] INTERGENERIC TRANSFER AND EXCHANGE RECOMBINATION OF RESTRICTION FRAGMENTS CLONED IN PBR322 - A NOVEL STRATEGY FOR THE REVERSED GENETICS OF THE TI PLASMIDS OF AGROBACTERIUM-TUMEFACIENS [J].

VANHAUTE, E ;

JOOS, H ;

MAES, M ;

WARREN, G ;

SCHELL, J ;

VANMONTAGU, M .

EMBO JOURNAL, 1983, 2 (03) :411-417

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