SURVIVAL OF AZOSPIRILLUM-BRASILENSE IN THE BULK SOIL AND RHIZOSPHERE OF 23 SOIL TYPES

被引:119
作者
BASHAN, Y
PUENTE, ME
RODRIGUEZMENDOZA, MN
TOLEDO, G
HOLGUIN, G
FERRERACERRATO, R
PEDRIN, S
机构
[1] CTR BIOL RES, DEPT MARINE RESOURCES, LA PAZ 23000, BAJA CALIF SUR, MEXICO
[2] COLEGIO POSTGRAD, NAT RESOURCES INST, MONTECILLO 56230, MEXICO, MEXICO
关键词
D O I
10.1128/AEM.61.5.1938-1945.1995
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The survival of Azospirillum brasilense Cd and Sp-245 in the rhizosphere of wheat and tomato plants and in 23 types of plant-free sterilized soils obtained from a wide range of environments in Israel and Mexico was evaluated. Large numbers of A. brasilense cells were detected in all the rhizospheres tested, regardless of soil type, bacterial strain, the origin of the soil, or the amount of rainfall each soil type received prior to sampling. Survival of A. brasilense in soils without plants differed from that in the rhizosphere and was mainly related to the geographical origin of the soil. In Israeli soils from arid, semiarid, or mountain regions, viability of A. brasilense rapidly declined or populations completely disappeared below detectable levels within 35 days after inoculation. In contrast, populations in the arid soils of Baja California Sur, Mexico, remained stable or even increased during the 45 day period after inoculation. In soils from Central Mexico, viability slowly decreased with time. In all soils, percentages of clay, nitrogen, organic matter, and water-holding capacity were positively correlated with bacterial viability. High percentages of CaCO3 and fine or rough sand had a highly negative effect on viability. The percentage of silt, pH, the percentage of phosphorus or potassium, electrical conductivity, and C/N ratio had no apparent effect on bacterial viability in the soil. Fifteen days after removal of inoculated plants, the remaining bacterial population in the three soil types tested began to decline sharply, reaching undetectable levels 90 days after inoculation. After plant removal, percolating the soils with water almost eliminated the A. brasilense population. Viability of A. brasilense in two artificial soils containing the same major soil components as the natural soils from Israel did was almost identical to that in the natural soils. We conclude that A. brasilense is a rhizosphere colonizer which survives poorly in most soils for prolonged periods of time; that outside the rhizosphere, seven abiotic parameters control the survival of this bacterium in the soil; and that disturbance of the soil (percolation with water or plant removal) directly and rapidly affects the population levels.
引用
收藏
页码:1938 / 1945
页数:8
相关论文
共 58 条
[41]   SUBSTRATE FLOW IN THE RHIZOSPHERE [J].
LYNCH, JM ;
WHIPPS, JM .
PLANT AND SOIL, 1990, 129 (01) :1-10
[42]   AZOSPIRILLUM - PLANT-ROOT ASSOCIATIONS - A REVIEW [J].
MICHIELS, K ;
VANDERLEYDEN, J ;
VANGOOL, A .
BIOLOGY AND FERTILITY OF SOILS, 1989, 8 (04) :356-368
[43]   COMPARING TREATMENT MEANS CORRECTLY AND APPROPRIATELY [J].
MIZE, CW ;
SCHULTZ, RC .
CANADIAN JOURNAL OF FOREST RESEARCH-REVUE CANADIENNE DE RECHERCHE FORESTIERE, 1985, 15 (06) :1142-1148
[44]   THE FATE OF MARKER AZOSPIRILLUM-LIPOFERUM INOCULATED INTO RICE AND ITS EFFECT ON GROWTH, YIELD AND N-2 FIXATION OF PLANTS STUDIED BY ACETYLENE-REDUCTION, N-15(2) FEEDING AND N-15 DILUTION TECHNIQUES [J].
NAYAK, DN ;
LADHA, JK ;
WATANABE, I .
BIOLOGY AND FERTILITY OF SOILS, 1986, 2 (01) :7-14
[45]  
Okon Yaacov, 1994, P274
[46]   PHYSIOLOGY, BIOCHEMISTRY, AND GENETICS OF AZOSPIRILLUM AND OTHER ROOT-ASSOCIATED NITROGEN-FIXING BACTERIA [J].
PEDROSA, FD .
CRC CRITICAL REVIEWS IN PLANT SCIENCES, 1988, 6 (04) :345-384
[47]   HABITABLE PORE-SPACE AND SURVIVAL OF RHIZOBIUM-LEGUMINOSARUM BIOVAR TRIFOLII INTRODUCED INTO SOIL [J].
POSTMA, J ;
VANVEEN, JA .
MICROBIAL ECOLOGY, 1990, 19 (02) :149-161
[48]  
PUENTE ME, 1993, SYMBIOSIS, V15, P49
[49]  
Ravikovitch S, 1992, SOILS ISRAEL FORMATI
[50]  
ROYCE CF, 1980, INTRO SEDIMENTARY AN, P180