CYTOSOLIC FREE MAGNESIUM, ATP, AND BLEBBING DURING CHEMICAL HYPOXIA IN CULTURED RAT HEPATOCYTES

被引：85

作者：

HARMAN, AW ^{[1
]}

NIEMINEN, AL ^{[1
]}

LEMASTERS, JJ ^{[1
]}

HERMAN, B ^{[1
]}

机构：

[1] UNIV N CAROLINA,SCH MED,DEPT CELL BIOL & ANAT,CELL BIOL LABS,CB 7090,CHAPEL HILL,NC 27599

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 170卷 / 02期

关键词：

D O I：

10.1016/0006-291X(90)92116-H

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cytosolic free Mg2+ concentration was determined in 1-day cultured rat hepatocytes using Multiparameter Digitized Video Microscopy (MDVM) of the fluorescent probe, magfura-2. Chemical hypoxia with KCN (5 mM) and iodoacetate (1 mM), a model which mimics the ATP depletion and reductive stress of hypoxia, caused a rapid increase of free Mg2+ from 1.1 ± 0.2 to 1.6 ± 0.2 mM within 4 min. Concurrently, numerous small plasma membrane blebs formed and ATP levels dropped from 13.24 to 1.32 nmol/106 cells. Removal of KCN and iodoacetate resulted in recovery of ATP to 60-70% of pre-exposure levels, a concomitant decrease in cytosolic free Mg2+ back toward basal levels, and reversal of blebbing (bleb resorption). These results indicate that changes of cytosolic free Mg2+ inversely reflect changes of ATP in a model of hypoxia and reoxygenation. Bleb formation and resorption were dependent on the fall and rise of ATP. © 1990.

引用

页码：477 / 483

页数：7

共 19 条

[1] Aikawa JK., 1991, MAGNESIUM ITS BIOL S, P21
[2] DIGUISEPPI J, 1985, Biotechniques, V3, P394
[3] EBEL H, 1980, J CLIN CHEM CLIN BIO, V18, P257
[4] GARFINKEL L, 1985, Magnesium, V4, P60
[5] INTRACELLULAR PH DURING CHEMICAL HYPOXIA IN CULTURED RAT HEPATOCYTES - PROTECTION BY INTRACELLULAR ACIDOSIS AGAINST THE ONSET OF CELL-DEATH
GORES, GJ
NIEMINEN, AL
WRAY, BE
HERMAN, B
LEMASTERS, JJ
[J]. JOURNAL OF CLINICAL INVESTIGATION, 1989, 83 (02) : 386 - 396
[6] IRREVERSIBLE INJURY IN ANOXIC HEPATOCYTES PRECIPITATED BY AN ABRUPT INCREASE IN PLASMA-MEMBRANE PERMEABILITY
HERMAN, B
NIEMINEN, AL
GORES, GJ
LEMASTERS, JJ
[J]. FASEB JOURNAL, 1988, 2 (02) : 146 - 151
[7] HERMAN B, 1990, IN PRESS CRC CRITICA
[8] JEWELL SA, 1982, SCIENCE, V217, P1257, DOI 10.1126/science.7112127
[9] BLEBBING, FREE CA-2+ AND MITOCHONDRIAL-MEMBRANE POTENTIAL PRECEDING CELL-DEATH IN HEPATOCYTES
LEMASTERS, JJ
DIGUISEPPI, J
NIEMINEN, AL
HERMAN, B
[J]. NATURE, 1987, 325 (6099) : 78 - 81
[10] CENTRILOBULAR INJURY FOLLOWING HYPOXIA IN ISOLATED, PERFUSED-RAT-LIVER
LEMASTERS, JJ
JI, S
THURMAN, RG
[J]. SCIENCE, 1981, 213 (4508) : 661 - 663

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