ELECTROPHORETIC PATTERN OF RODENT SEMINAL-VESICLE PROTEINS AS REVEALED BY SILVER STAINING

被引：10

作者：

CARBALLADA, R

ESPONDA, P

机构：

[1] Centro de Investigaciones Biológicas, CSIC, Madrid

来源：

INTERNATIONAL JOURNAL OF ANDROLOGY | 1991年 / 14卷 / 01期

关键词：

GEL ELECTROPHORESIS; SEMEN; SEMINAL PLASMA; SEMINAL VESICLES; SILVER STAIN;

D O I：

10.1111/j.1365-2605.1991.tb01062.x

中图分类号：

R69 [泌尿科学（泌尿生殖系疾病）];

学科分类号：

摘要：

Electrophoresis of seminal vesicle secretions (SVS) from several rodents showed a very simple pattern composed of 3-5 main protein bands when an anionic dye (Coomassie brilliant blue) was used. However, use of a silver staining method showed a more complex protein spectrum, and several minor components of 12-90 kD, were clearly revealed. Western blotting using antibodies to SVS demonstrated that these minor protein components were not serum contaminants. Rat, mouse and hamster SVS shared antigenic determinants which were not related to rabbit SVS.

引用

页码：52 / 57

页数：6