CHLORAMPHENICOL RESISTANCE PLASMIDS IN STAPHYLOCOCCUS-AUREUS ISOLATED FROM BOVINE SUBCLINICAL MASTITIS

Chloramphenicol resistance (Cm(R)) could be detected in 11 of 217 Staphylococcus aureus isolates from bovine subclinical mastitis. All isolates were assigned to biotypes A or C. The Cm(R)-determinants were found to be located exclusively on small plasmids of approximately 4.6 kb as revealed by protoplast transformation. The 11 Cm(R)-plasmids could be differentiated on the basis of restriction endonuclease analyses. The restriction maps of these Cm(R)-plasmids identified two separate groups. One group demonstrated homology to the plasmid pC 221, the other to the plasmid pC 223. Both prototype plasmids, pC 221 and pC 223, had been isolated from S. aureus of human origin.