MUTATIONAL ANALYSIS OF CANDIDATE GENES IN PSYCHIATRIC-DISORDERS

被引：11

作者：

GEJMAN, PV ^{[1
]}

GELERNTER, J ^{[1
]}

机构：

[1] YALE UNIV, SCH MED,W HAVEN VET ADM MED CTR,DEPT PSYCHIAT, DIV MOLEC PSYCHIAT, West Haven, CT 06516 USA

来源：

AMERICAN JOURNAL OF MEDICAL GENETICS | 1993年 / 48卷 / 04期

关键词：

DENATURING GRADIENT GEL ELECTROPHORESIS; SINGLE STRANDED CONFORMATIONAL POLYMORPHISMS; CHEMICAL CLEAVAGE; ASSOCIATION;

D O I：

10.1002/ajmg.1320480404

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A genetic hypothesis for a disease presupposes the existence of variation in the DNA sequences of affected individuals. A series of techniques known together as ''mutational analysis'' can be applied towards identifying new sequence variations in selected genes. These techniques can screen a large series of individuals for mutations efficiently, so it is not necessary to determine the nucleotide sequence in every DNA sample. DNA samples suspected of harboring sequence variants are then sequenced. Denaturing gradient gel electrophoresis techniques, single stranded conformation polymorphism paradigms, and chemical cleavage of mismatches are 3 procedures widely used for the molecular screening of mutations today. We discuss each of these techniques for mutation screening. (C) 1993 Wiley-Liss, Inc.

引用

页码：184 / 191

页数：8

共 60 条

[41] TEMPERATURE-GRADIENT GEL-ELECTROPHORESIS OF NUCLEIC-ACIDS - ANALYSIS OF CONFORMATIONAL TRANSITIONS, SEQUENCE VARIATIONS, AND PROTEIN-NUCLEIC ACID INTERACTIONS [J].