TRANSFORMING GROWTH-FACTOR-ALPHA IN HUMAN SUBMANDIBULAR-GLAND AND SALIVA

被引：12

作者：

MOGI, M

INAGAKI, H

KOJIMA, K

MINAMI, M

HARADA, M

机构：

[1] NIPPON MED COLL,DEPT HYG & PUBL HLTH,TOKYO 113,JAPAN

[2] FOOD & DRUG SAFETY CTR,HATANO RES INST,HADANO 257,JAPAN

来源：

JOURNAL OF IMMUNOASSAY | 1995年 / 16卷 / 04期

关键词：

TGF-ALPHA; EGF; EIA; SUBMANDIBULAR GLAND;

D O I：

10.1080/15321819508013569

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A sensitive sandwich enzyme immunoassay (ELA) for transforming growth factor-alpha (TGF-alpha) utilizing a polyclonal antibody that recognizes limited epitopes of both human TGF-alpha and rat TGF-alpha in combination with a monoclonal anti-TGF-alpha IgG(1) galactosidase conjugate was developed. This assay shows no crossreactivity with human epidermal growth factor, We can quantify the TGF-alpha level in not only human TGF-alpha (detection limit: 1 pg/ml), but also rat TGF-alpha (detection limit: 10 pg/ml) by virtue of cross-reactivity. Employing this assay system, we demonstrated that TGF-alpha is present in both human submandibular glands and submandibular/sublingual saliva.

引用

收藏

页码：379 / 394

页数：16

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