MULTIPRINS: a method for multicolour primed in situ labelling

被引：16

作者：

Volpi, E. V. ^{[1
,2
]}

Baldini, A. ^{[3
]}

机构：

[1] Imperial Canc Res Fund, London WC2A 3PX, England

[2] Univ Rome La Sapienza, Dipartimento Genet & Biol Mol, I-00185 Rome, Italy

[3] Baylor Coll Med, Inst Mol Genet, Houston, TX 77030 USA

来源：

CHROMOSOME RESEARCH | 1993年 / 1卷 / 04期

关键词：

fluorescence labelling; MULTIPRINS; repetitive DNA mapping;

D O I：

10.1007/BF00710131

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

With this fast and simple MULTIPRINS protocol we show simultaneous mapping of multiple repetitive sequences by sequential cycles of primed in situ (PRINS) labelling with various primers and reporter molecules. Differentially labelled DNA sequences synthesized in situ were visualized by directly incorporated rhodamine-4-dUTP, by fluorescein isothiocyanate and Cy5 using digital imaging microscopy. This further development of the PRINS method enhances its potential as an alternative to traditional in situ hybridization.

引用

页码：257 / 260

页数：4

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