SEQUENCE ORGANIZATION OF THE BALBIANI-RING-2.1 GENE IN CHIRONOMUS-TENTANS

被引:23
作者
WIESLANDER, L
PAULSSON, G
机构
[1] Department of Molecular Genetics, Medical Nobel Institute, Karolinska Institutet
关键词
GENE FAMILY; REPETITIVE SEQUENCES; EXON-INTRON STRUCTURE;
D O I
10.1073/pnas.89.10.4578
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Balbiani rings are giant chromosomal puffs, containing related genes that provide unique possibilities for in vivo analysis of gene expression at the chromatin and ribonucleoprotein levels. Here, the 5' end of the Balbiani ring 2.1 gene in the dipteran Chironomus tentans has been isolated and the sequence organization of the entire Balbiani ring gene is presented. The gene contains five exons, one being extremely small, only 6 base pairs, and one being extremely large, almost-equal-to 30 kilobase pairs. Three introns are located at the 5' end and a fourth one is located at the 3' end. The central 30-kilobase-pair exon is entirely built from tandemly organized repeats. All repeats are virtually identical except for a few variant repeats at both ends of the repeat array. The number of repeats may vary between alleles and the length of the gene therefore changes between 30 and 35 kilobase pairs.
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页码:4578 / 4582
页数:5
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