HUMAN DNA HELICASE-IV IS NUCLEOLIN, AN RNA HELICASE MODULATED BY PHOSPHORYLATION

被引：82

作者：

TUTEJA, N ^{[1
]}

HUANG, NW ^{[1
]}

SKOPAC, D ^{[1
]}

TUTEJA, R ^{[1
]}

HRVATIC, S ^{[1
]}

ZHANG, JW ^{[1
]}

PONGOR, S ^{[1
]}

JOSEPH, G ^{[1
]}

FAUCHER, C ^{[1
]}

AMALRIC, F ^{[1
]}

FALASCHI, A ^{[1
]}

机构：

[1] CNRS,CTR RECH BIOCHIM & GENET CELLULAIRES,F-31062 TOULOUSE,FRANCE

来源：

GENE | 1995年 / 160卷 / 02期

关键词：

UNWINDING ENZYME; CDC2; CASEIN KINASE; NUCLEOLUS; PRE-RIBOSOME ASSEMBLY;

D O I：

10.1016/0378-1119(95)00207-M

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The cDNA encoding human DNA helicase IV (HDH IV), a 100-kDa protein which unwinds DNA in the 5' to 3' direction with respect to the bound strand, was cloned and sequenced. It was found to be identical to the human cDNA encoding nucleolin, a ubiquitous eukaryotic protein essential for pre-ribosome assembly. HDH IV/nucleolin can unwind RNA-RNA duplexes, as well as DNA-DNA and DNA-RNA duplexes. Phosphorylation of HDH IV/nucleolin by cdc2 kinase and casein kinase II enhanced its unwinding activity in an additive way. The Gly-rich C-terminal domain possesses a limited ATP-dependent duplex-unwinding activity which contributes to the helicase activity of HDH IV/nucleolin.

引用

页码：143 / 148

页数：6

共 20 条

[1] MITOSIS-SPECIFIC PHOSPHORYLATION OF NUCLEOLIN BY P34CDC2 PROTEIN-KINASE [J].

BELENGUER, P ;

CAIZERGUESFERRER, M ;

LABBE, JC ;

DOREE, M ;

AMALRIC, F .

MOLECULAR AND CELLULAR BIOLOGY, 1990, 10 (07) :3607-3618

[2]

CAIZERGUESFERRE.M, 1987, BIOCHEMISTRY-US, V26, P7876

[3]

FLORESROZAS H, 1993, J BIOL CHEM, V268, P21372

[4] CONCERTED ACTIVITIES OF THE RNA RECOGNITION AND THE GLYCINE-RICH C-TERMINAL DOMAINS OF NUCLEOLIN ARE REQUIRED FOR EFFICIENT COMPLEX-FORMATION WITH PRERIBOSOMAL RNA [J].

GHISOLFI, L ;

KHARRAT, A ;

JOSEPH, G ;

AMALRIC, F ;

ERARD, M .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1992, 209 (02) :541-548

[5]

GHISOLFI L, 1992, J BIOL CHEM, V267, P2955

[6]

HARLOW E, 1988, ANTIBODIES LABORATOR

[7] DNA HELICASES - ENZYMES WITH ESSENTIAL ROLES IN ALL ASPECTS OF DNA METABOLISM [J].

MATSON, SW ;

BEAN, DW ;

GEORGE, JW .

BIOESSAYS, 1994, 16 (01) :13-22

[8] U20, A NOVEL SMALL NUCLEOLAR RNA, IS ENCODED IN AN INTRON OF THE NUCLEOLIN GENE IN MAMMALS [J].

NICOLOSO, M ;

CAIZERGUESFERRER, M ;

MICHOT, B ;

AZUM, MC ;

BACHELLERIE, JP .

MOLECULAR AND CELLULAR BIOLOGY, 1994, 14 (09) :5766-5776

[9] INTERACTION OF NUCLEOLAR PHOSPHOPROTEIN-C23 WITH CLONED SEGMENTS OF RAT RIBOSOMAL DEOXYRIBONUCLEIC-ACID [J].

OLSON, MOJ ;

RIVERS, ZM ;

THOMPSON, BA ;

KAO, WY ;

CASE, ST .

BIOCHEMISTRY, 1983, 22 (14) :3345-3351

[10]

Sambrook J., 1989, MOL CLONING LAB MANU

← 1 2 →