LIGHT-HARVESTING COMPLEXES OF BROWN-ALGAE - BIOCHEMICAL-CHARACTERIZATION AND IMMUNOLOGICAL RELATIONSHIPS

被引：41

作者：

PASSAQUET, C

THOMAS, JC

CARON, L

HAUSWIRTH, N

PUEL, F

BERKALOFF, C

机构：

[1] Laboratoire des Biomembranes et Surfaces Cellulaires Vègètales, UA CNRS DO311, Ecole Normale Supèrieure

来源：

FEBS LETTERS | 1991年 / 280卷 / 01期

关键词：

BROWN ALGAE; FUCOXANTHIN; LIGHT-HARVESTING COMPLEX; POLYCLONAL ANTIBODY;

D O I：

10.1016/0014-5793(91)80195-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The pigment composition of the light-harvesting complexes isolated from several brown algae belonging to different orders has been analysed by reverse-phase HPLC. Relative to whole chloroplasts, they were markedly enriched in Chl c, fucoxanthin and violaxanthin and conversely depleted in Chl a. The relative molar proportions of the 4 main pigments (Chl a/Chl c/fucoxanthin/violaxanthin) ranged from 100:18:76:6 to 100:30:107:17. The protein moiety of LH complexes of all the species studied were composed of one or two main polypeptide components in the range of 19-22 kDa. These polypeptide subunits were arranged in polymeric particles about 240 kDa in Laminaria saccharina. A polyclonal antibody raised against the LH polypeptide of Fucus serratus has been tested on LH apoproteins of other Chromophytes and Chlorophytes. Phylogenic inplications of these results are discussed.

引用

页码：21 / 26

页数：6

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