DETERMINATION OF NICOTINE AND 2 MAJOR METABOLITES IN SERUM BY SOLID-PHASE EXTRACTION AND HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY, AND HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY PARTICLE BEAM MASS-SPECTROMETRY

被引：47

作者：

PACIFICI, R

PICHINI, S

ALTIERI, I

ROSA, M

BACOSI, A

CARONNA, A

ZUCCARO, P

机构：

[1] IST SUPER SANITA,VIALE REGINA ELENA 299,I-00161 ROME,ITALY

[2] OSPED SANTO SPIRITO,ROME,ITALY

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1993年 / 612卷 / 02期

关键词：

D O I：

10.1016/0378-4347(93)80165-Z

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A rapid and selective assay of nicotine, cotinine and trans-3'-hydroxycotinine in human serum, based on high-performance liquid chromatography with UV detection has been developed. The compounds were subjected to solid-phase extraction, using Extrelut 1 cartridges. Recoveries were ca. 95% for nicotine, 90% for cotinine and 50-55% for trans-3'-hydroxycotinine. The limit of quantitation observed with this method was 10 ng/ml for nicotine and 5 ng/ml for each of the metabolites. The compounds were also identified using high-performance liquid chromatography with particle beam mass spectrometry, to confirm their presence in human serum.

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页码：209 / 213

页数：5

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