TRANSFORMATION OF COTTON (GOSSYPIUM-HIRSUTUM-L) VIA PARTICLE BOMBARDMENT

被引：169

作者：

FINER, JJ

MCMULLEN, MD

机构：

[1] OHIO STATE UNIV,USDA ARS,OHIO STATE BIOTECHNOL CTR,WOOSTER,OH 44691

[2] OHIO STATE UNIV,USDA ARS,CORN & SOYBEAN RES UNIT,WOOSTER,OH 44691

[3] OHIO STATE UNIV,USDA ARS,OHIO AGR RES & DEV CTR,WOOSTER,OH 44691

来源：

PLANT CELL REPORTS | 1990年 / 8卷 / 10期

关键词：

D O I：

10.1007/BF00270059

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Embryogenic suspension cultures of cotton (Gossypium hirsutum L.) were subjected to particle bombardment, where high density particles carrying plasmid DNA were accelerated towards the embryogenic plant cells. The plasmid DNA coating the particles encoded hygromycin resistance. One to two weeks following bombardment, embryogenic cotton cells were placed in proliferation medium containing 100 μg/ml hygromycin. Clumps of tissue which grew in the presence of hygromycin were subcultured at low density into fresh hygromycin-containing proliferation medium. Following sequential transfer of embryogenic tissue to development and then germination media, plants were recovered from transgenic embryogenic tissue. Southern hybridization confirmed the presence of the hygromycin resistance gene in embryogenic suspension culture tissue and regenerated plants. © 1990 Springer-Verlag.

引用

页码：586 / 589

页数：4

共 19 条

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