REVERSAL OF DRUG-RESISTANCE BY CYCLOSPORINE-A IN A PATIENT WITH ACUTE MYELOCYTIC-LEUKEMIA

被引：95

作者：

SONNEVELD, P ^{[1
]}

NOOTER, K ^{[1
]}

机构：

[1] TNO,IIRI,INST APPL RADIOBIOL & IMMUNOL,RIJSWIJK,NETHERLANDS

来源：

BRITISH JOURNAL OF HAEMATOLOGY | 1990年 / 75卷 / 02期

关键词：

D O I：

10.1111/j.1365-2141.1990.tb02650.x

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

The emergence of resistant leukaemia in a patient with acute myeloid leukaemia (AML) was evaluated during clinical progression of the disease. At relapse, a decrease of the intracellular accumulation of daunorubicin (DNR) as determined by real time flow cytometry was associated with a relative overexpression of RNA encoding for the multidrug resistance phenotype (MDR1), and by a decreased in vitro sensitivity to DNR of clonogenic AML cells (IC50 0.8‐3.4 μm). Intracellular DNR accumulation and in vitro DNR sensitivity could be completely restored by adding cyclosporin‐A (3 μm) to the cells. At progressive relapse the patient was treated with re‐induction therapy (DNR 30 mg/m2 x 3. cytarabine 200 mg/m2 x 7) to which cyclosporin‐A was added (Cy‐A 4 mg/kg twice daily for 3 d, 2.5 mg/kg twice daily for 2 d). which resulted in elimination of the MDR1 positive AML cells with restoration of the original DNR accumulation and in vitro sensitivity. After 12 weeks the resistant clone reappeared in the blood and bone marrow. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：208 / 211

页数：4