IDENTIFICATION OF HTLV-I SEQUENCE IN CORD BLOOD MONONUCLEAR-CELLS OF NEONATES BORN TO HTLV-I ANTIGEN ANTIBODY-POSITIVE MOTHERS BY POLYMERASE CHAIN-REACTION

被引：30

作者：

SAITO, S

FURUKI, K

ANDO, Y

TANIGAWA, T

KAKIMOTO, K

MORIYAMA, I

ICHIJO, M

机构：

[1] Department of Obstetrics and Gynecology, Nara Medical University, Nara, 634, 840 Shijo‐cho, Kashihara

来源：

JAPANESE JOURNAL OF CANCER RESEARCH | 1990年 / 81卷 / 09期

关键词：

Cord blood; HTLV‐I; Intrauterine infection; Polymerase chain reaction;

D O I：

10.1111/j.1349-7006.1990.tb02663.x

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

We developed a polymerase chain reaction (PCR) method which has high sensitivity and simple technique in order to investigate the presence or absence of human T lymphotropic virus type I (HTLV‐I) provirus in cord blood mononuclear cells of neonates born to HTLV‐I carrier mothers. Out of 40, three subjects were found to contain the HTLV‐I provirus genome. These three subjects remained HTLV‐I sequence‐positive in follow‐up study. On the other hand, when examined by a conventional technique for detection of HTLV‐I‐associated antigen on peripheral mononuclear cells, all 40 neonates were HTLV‐I‐associated antigen‐negative. These results suggest that PCR is more sensitive than the conventional antigen detection method and is useful in early detection of HTLV‐I infection in neonates born to HTLV‐I carriers. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：890 / 895

页数：6

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