USE OF AN OPERON FUSION TO INDUCE EXPRESSION AND CRYSTALLIZATION OF A BACILLUS-THURINGIENSIS DELTA-ENDOTOXIN ENCODED BY A CRYPTIC GENE

被引：26

作者：

CRICKMORE, N ^{[1
]}

WHEELER, VC ^{[1
]}

ELLAR, DJ ^{[1
]}

机构：

[1] UNIV CAMBRIDGE,DEPT BIOCHEM,CAMBRIDGE CB2 1QW,ENGLAND

来源：

MOLECULAR & GENERAL GENETICS | 1994年 / 242卷 / 03期

基金：

英国惠康基金;

关键词：

CRYIIB; DELTA-ENDOTOXIN; CRYPTIC GENE; OPERON; CHAPERONE;

D O I：

10.1007/BF00280428

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A delta-endotoxin gene previously cloned from Bacillus thuringiensis subsp. galleriae has been shown by a combination of restriction mapping and DNA sequence analysis to be a cryIIB clone; in common with other cyIIB genes it was found to lack a functional promoter. Addition of a promoter resulted in expression of the gene in Bacillus thuringiensis but did not result in the formation of the crystalline inclusions normally associated with such toxins. Inclusion formation was only observed when the gene was incorporated into an operon containing a gene known to be involved in the crystallisation of another delta-endotoxin.

引用

页码：365 / 368

页数：4

共 12 条

[1] A 20-KILODALTON PROTEIN IS REQUIRED FOR EFFICIENT PRODUCTION OF THE BACILLUS-THURINGIENSIS SUBSP ISRAELENSIS 27-KILODALTON CRYSTAL PROTEIN IN ESCHERICHIA-COLI [J].