H-2-FORMING N-5,N-10-METHYLENETETRAHYDROMETHANOPTERIN DEHYDROGENASE - MECHANISM OF H-2 FORMATION ANALYZED USING HYDROGEN ISOTOPES

被引：30

作者：

KLEIN, AR

FERNANDEZ, VM

THAUER, RK

机构：

[1] UNIV MARBURG,FACHBEREICH BIOL,MIKROBIOL LAB,D-35043 MARBURG,GERMANY

[2] MAX PLANCK INST TERR MIKROBIOL,D-35043 MARBURG,GERMANY

[3] UNIV AUTONOMA MADRID,CSIC,INST CATALISIS,UNIDAD BIOCATALISIS,E-28049 MADRID,SPAIN

来源：

FEBS LETTERS | 1995年 / 368卷 / 02期

关键词：

HYDROGENASE; TETRAHYDROMETHANOPTERIN; ENZYME MECHANISM; METHANOGENIC ARCHAEA; METHANOBACTERIUM THERMOAUTOTROPHICUM;

D O I：

10.1016/0014-5793(95)00642-M

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

H-2-forming N-5,N-10-methylenetetrahydromethanopterin dehydrogenase catalyzes the reversible dehydrogenation of N-5,N-10-methylenetetrahydromethanopterin (CH2 = H(4)MPT) to N-5,N-10-methenyltetrahydromethanopterin (CH = H(4)MPT(+)) and H-2. In D2O both HD and D-2 are formed from CH2 = H(4)MPT and in H2O both HD and H-2 from CD2 = H(4)MPT. Evidence is presented that HD is not an intermediate in the formation of D-2 and H-2, respectively.

引用

页码：203 / 206

页数：4

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