COLOCALIZATION OF CALCIUM-ENTRY AND EXOCYTOTIC RELEASE SITES IN ADRENAL CHROMAFFIN CELLS

被引：129

作者：

ROBINSON, IM ^{[1
]}

FINNEGAN, JM ^{[1
]}

MONCK, JR ^{[1
]}

WIGHTMAN, RM ^{[1
]}

FERNANDEZ, JM ^{[1
]}

机构：

[1] UNIV N CAROLINA,DEPT CHEM,CHAPEL HILL,NC 27599

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1995年 / 92卷 / 07期

关键词：

PULSED-LASER CA2+ IMAGING; AMPEROMETRY; SECRETION; ACTIVE ZONES;

D O I：

10.1073/pnas.92.7.2474

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

''Snapshot'' images of localized Ca2+ influx into patch-clamped chromaffin cells were captured by using a recently developed pulsed-laser imaging system. Transient opening of voltage-sensitive Ca2+ channels gave rise to localized elevations of Ca2+ that had the appearance of either ''hotspots'' or partial rings found immediately beneath the plasma membrane. When the Ca2+ imaging technique was employed in conjunction with flame-etched carbon-fiber electrodes to spatially map the release sites of catecholamines, it was observed that the sites of Ca2+ entry and catecholamine release were colocalized. These results provide functional support for the idea that secretion occurs from ''active zone''-like structures in neuroendocrine cells.

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页码：2474 / 2478

页数：5

相关论文

共 40 条

[1] 3 TYPES OF CA2+ CHANNEL TRIGGER SECRETION WITH DIFFERENT EFFICACIES IN CHROMAFFIN CELLS [J].

ARTALEJO, CR ;

ADAMS, ME ;

FOX, AP .

NATURE, 1994, 367 (6458) :72-76

[2] THE MOLECULAR MACHINERY FOR SECRETION IS CONSERVED FROM YEAST TO NEURONS [J].

BENNETT, MK ;

SCHELLER, RH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (07) :2559-2563

[3] SYNTAXIN - A SYNAPTIC PROTEIN IMPLICATED IN DOCKING OF SYNAPTIC VESICLES AT PRESYNAPTIC ACTIVE ZONES [J].

BENNETT, MK ;

CALAKOS, N ;

SCHELLER, RH .

SCIENCE, 1992, 257 (5067) :255-259

[4] A MAJOR ROLE FOR PROTEIN KINASE-C IN CALCIUM-ACTIVATED EXOCYTOSIS IN PERMEABILISED ADRENAL CHROMAFFIN CELLS [J].

BURGOYNE, RD ;

MORGAN, A ;

OSULLIVAN, AJ .

FEBS LETTERS, 1988, 238 (01) :151-155

[5] CONTROL OF EXOCYTOSIS IN ADRENAL CHROMAFFIN CELLS [J].

BURGOYNE, RD .

BIOCHIMICA ET BIOPHYSICA ACTA, 1991, 1071 (02) :174-202

[6] ULTRASTRUCTURAL DEMONSTRATION OF EXOCYTOSIS IN THE INTACT RAT ADRENAL-MEDULLA [J].

CARMICHAEL, SW ;

BROOKS, JC ;

MALHOTRA, RK ;

WAKADE, TD ;

WAKADE, AR .

JOURNAL OF ELECTRON MICROSCOPY TECHNIQUE, 1989, 12 (04) :316-322

[7] SPATIAL LOCALIZATION OF THE STIMULUS-INDUCED RISE IN CYTOSOLIC CA-2+ IN BOVINE ADRENAL CHROMAFFIN CELLS - DISTINCT NICOTINIC AND MUSCARINIC PATTERNS [J].

CHEEK, TR ;

OSULLIVAN, AJ ;

MORETON, RB ;

BERRIDGE, MJ ;

BURGOYNE, RD .

FEBS LETTERS, 1989, 247 (02) :429-434

[8] SIMULTANEOUS MEASUREMENTS OF CYTOSOLIC CALCIUM AND SECRETION IN SINGLE BOVINE ADRENAL CHROMAFFIN CELLS BY FLUORESCENT IMAGING OF FURA-2 IN COCULTURED CELLS [J].

CHEEK, TR ;

JACKSON, TR ;

OSULLIVAN, AJ ;

MORETON, RB ;

BERRIDGE, MJ ;

BURGOYNE, RD .

JOURNAL OF CELL BIOLOGY, 1989, 109 (03) :1219-1227

[9] DELAY IN VESICLE FUSION REVEALED BY ELECTROCHEMICAL MONITORING OF SINGLE SECRETORY EVENTS IN ADRENAL CHROMAFFIN CELLS [J].

CHOW, RH ;

VONRUDEN, L ;

NEHER, E .

NATURE, 1992, 356 (6364) :60-63

[10]

COUTEAUX R, 1970, Comptes Rendus Hebdomadaires des Seances de l'Academie des Sciences Serie D Sciences Naturelles, V271, P2346

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