COLOCALIZATION OF CALCIUM-ENTRY AND EXOCYTOTIC RELEASE SITES IN ADRENAL CHROMAFFIN CELLS

被引：129

作者：

ROBINSON, IM ^{[1
]}

FINNEGAN, JM ^{[1
]}

MONCK, JR ^{[1
]}

WIGHTMAN, RM ^{[1
]}

FERNANDEZ, JM ^{[1
]}

机构：

[1] UNIV N CAROLINA,DEPT CHEM,CHAPEL HILL,NC 27599

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1995年 / 92卷 / 07期

关键词：

PULSED-LASER CA2+ IMAGING; AMPEROMETRY; SECRETION; ACTIVE ZONES;

D O I：

10.1073/pnas.92.7.2474

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

''Snapshot'' images of localized Ca2+ influx into patch-clamped chromaffin cells were captured by using a recently developed pulsed-laser imaging system. Transient opening of voltage-sensitive Ca2+ channels gave rise to localized elevations of Ca2+ that had the appearance of either ''hotspots'' or partial rings found immediately beneath the plasma membrane. When the Ca2+ imaging technique was employed in conjunction with flame-etched carbon-fiber electrodes to spatially map the release sites of catecholamines, it was observed that the sites of Ca2+ entry and catecholamine release were colocalized. These results provide functional support for the idea that secretion occurs from ''active zone''-like structures in neuroendocrine cells.

引用

页码：2474 / 2478

页数：5

共 40 条

[31] CALCIUM GRADIENTS AND BUFFERS IN BOVINE CHROMAFFIN CELLS [J].

NEHER, E ;

AUGUSTINE, GJ .

JOURNAL OF PHYSIOLOGY-LONDON, 1992, 450 :273-301

[32] DISCRETE CHANGES OF CELL-MEMBRANE CAPACITANCE OBSERVED UNDER CONDITIONS OF ENHANCED SECRETION IN BOVINE ADRENAL CHROMAFFIN CELLS [J].

NEHER, E ;

MARTY, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1982, 79 (21) :6712-6716

[33] VISUALIZATION OF THE EXOCYTOSIS ENDOCYTOSIS SECRETORY CYCLE IN CULTURED ADRENAL CHROMAFFIN CELLS [J].

PHILLIPS, JH ;

BURRIDGE, K ;

WILSON, SP ;

KIRSHNER, N .

JOURNAL OF CELL BIOLOGY, 1983, 97 (06) :1906-1917

[34] ARE THE PRE-SYNAPTIC MEMBRANE-PARTICLES THE CALCIUM CHANNELS [J].

PUMPLIN, DW ;

REESE, TS ;

LLINAS, R .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1981, 78 (11) :7210-7213

[35]

Robinson Iain M., 1994, Current Opinion in Neurobiology, V4, P330, DOI 10.1016/0959-4388(94)90093-0

[36]

SCHROEDER TJ, 1994, J BIOL CHEM, V269, P17215

[37] CHARACTERIZATION OF SUBMICRON-SIZED CARBON ELECTRODES INSULATED WITH A PHENOL ALLYLPHENOL COPOLYMER [J].

STREIN, TG ;

EWING, AG .

ANALYTICAL CHEMISTRY, 1992, 64 (13) :1368-1373

[38] ROLES OF N-TYPE AND Q-TYPE CA2+ CHANNELS IN SUPPORTING HIPPOCAMPAL SYNAPTIC TRANSMISSION [J].

WHEELER, DB ;

RANDALL, A ;

TSIEN, RW .

SCIENCE, 1994, 264 (5155) :107-111

[39] TEMPORALLY RESOLVED CATECHOLAMINE SPIKES CORRESPOND TO SINGLE VESICLE RELEASE FROM INDIVIDUAL CHROMAFFIN CELLS [J].

WIGHTMAN, RM ;

JANKOWSKI, JA ;

KENNEDY, RT ;

KAWAGOE, KT ;

SCHROEDER, TJ ;

LESZCZYSZYN, DJ ;

NEAR, JA ;

DILIBERTO, EJ ;

VIVEROS, OH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (23) :10754-10758

[40] PARALYSIS AND EARLY DEATH IN CYSTEINE STRING PROTEIN MUTANTS OF DROSOPHILA [J].

ZINSMAIER, KE ;

EBERLE, KK ;

BUCHNER, E ;

WALTER, N ;

BENZER, S .

SCIENCE, 1994, 263 (5149) :977-980

← 1 2 3 4 →