BINDING BETWEEN WILD-TYPE P53 AND HSP72 ACCUMULATED AFTER UV AND GAMMA-RAY IRRADIATION

被引：66

作者：

MATSUMOTO, H ^{[1
]}

WANG, XJ ^{[1
]}

OHNISHI, T ^{[1
]}

机构：

[1] NARA MED UNIV,DEPT BIOL,KASHIHARA,NARA 634,JAPAN

来源：

CANCER LETTERS | 1995年 / 92卷 / 02期

关键词：

P53; PROTEIN; HEAT SHOCK PROTEIN; ULTRA VIOLET IRRADIATION; GAMMA-RAY IRRADIATION;

D O I：

10.1016/0304-3835(95)03769-S

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

We have investigated the increased levels of p53 and hsp72 after UV or gamma-ray irradiation and the association of these using two human glioblastoma cell lines. Human glioblastoma cell line A-172 exhibited no mutations in the p53 gene, whereas cell line T98G had a mutation in exon 7 of the p53 gene. In A-172, the level of wild-type p53 was increased by UV or gamma-ray irradiation. Although the level of mutant p53 in T98G was very high before irradiation, it was unchanged by UV or gamma-ray irradiation. Furthermore, in the A-172 cell line after UV or gamma-ray irradiation, wild-type p53 was co-immunoprecipitated with anti-hsp72/73 antibody, and accumulated hsp72 was also co-immunoprecipitated with anti-p53 antibody. These findings indicate that wild-type p53 accumulated by UV or gamma-ray irradiation is associated with hsp72 induced by these treatments.

引用

页码：127 / 133

页数：7

共 26 条

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