PHOTOCHEMISTRY OF RHODOPSIN AND ISORHODOPSIN INVESTIGATED ON A PICOSECOND TIME SCALE

被引:53
作者
MONGER, TG [1 ]
ALFANO, RR [1 ]
CALLENDER, RH [1 ]
机构
[1] CUNY CITY COLL, DEPT PHYS, NEW YORK, NY 10031 USA
关键词
D O I
10.1016/S0006-3495(79)85205-4
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Bovine rhodopsin and isorhodopsin were excited with a single 530 nm, 7 ps light pulse emitted by a mode-locked Nd3+ glass laser at room temperature. Within 3 ps of excitation, absorbance changes due to formation of bathorhodopsin were observed. The difference spectra generated during and 100 ps after pulse excitation are presented. Bathorhodopsin formation is completed within 3 ps for both primary pigments. A single common bathorhodopsin is apparently photochemically formed from both primary pigments. These findings provide additional support for the cis-trans isomerization model of the primary event in vision. Additional absorption transients that were observed near 670 and 460 nm are discussed.
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页码:105 / 115
页数:11
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