PHOTOCHEMISTRY OF RHODOPSIN AND ISORHODOPSIN INVESTIGATED ON A PICOSECOND TIME SCALE

被引：53

作者：

MONGER, TG ^{[1
]}

ALFANO, RR ^{[1
]}

CALLENDER, RH ^{[1
]}

机构：

[1] CUNY CITY COLL, DEPT PHYS, NEW YORK, NY 10031 USA

来源：

BIOPHYSICAL JOURNAL | 1979年 / 27卷 / 01期

关键词：

D O I：

10.1016/S0006-3495(79)85205-4

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Bovine rhodopsin and isorhodopsin were excited with a single 530 nm, 7 ps light pulse emitted by a mode-locked Nd3+ glass laser at room temperature. Within 3 ps of excitation, absorbance changes due to formation of bathorhodopsin were observed. The difference spectra generated during and 100 ps after pulse excitation are presented. Bathorhodopsin formation is completed within 3 ps for both primary pigments. A single common bathorhodopsin is apparently photochemically formed from both primary pigments. These findings provide additional support for the cis-trans isomerization model of the primary event in vision. Additional absorption transients that were observed near 670 and 460 nm are discussed.

引用

页码：105 / 115

页数：11