CLONING AND SEQUENCE-ANALYSIS OF THE FLAGELLIN GENE OF CAMPYLOBACTER-JEJUNI TGH9011

被引：21

作者：

KHAWAJA, R ^{[1
]}

NEOTE, K ^{[1
]}

BINGHAM, HL ^{[1
]}

PENNER, JL ^{[1
]}

CHAN, VL ^{[1
]}

机构：

[1] UNIV TORONTO,DEPT MICROBIOL,FITZGERALD BLDG,150 COLL ST,TORONTO M5S 1A8,ONTARIO,CANADA

来源：

CURRENT MICROBIOLOGY | 1992年 / 24卷 / 04期

关键词：

D O I：

10.1007/BF01579284

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Flagella are essential for motility and have been implicated to be one of the pathogenic determinants. The flagellum of Campylobacter jejuni is a polymeric structure of a 62-kd protein. Using a high-affinity flagellin antibody to screen a lambda gt11 phage genomic expression library of C. jejuni strain TGH9011 (Serotype LIO36), a recombinant phage clone lambda gt11RK that expresses the C. jejuni flagellin protein was isolated. The recombinant lambda gt11RK produced a 56-kd protein upon induction with isopropylthiogalactoside, which reacted specifically with anti-flagellin antibody. The flagellin gene was sequenced, and comparative analysis of the nucleotide and amino acid sequence identified a region of the flagellin that shows hypervariability among different Campylobacter species and strains.

引用

页码：213 / 221

页数：9

共 45 条

[41] COVALENT STRUCTURE OF 3 PHASE-1 FLAGELLAR FILAMENT PROTEINS OF SALMONELLA
WEI, LN
JOYS, TM
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1985, 186 (04) : 791 - 803
[42] ANTIGENIC ANALYSIS OF CAMPYLOBACTER FLAGELLAR PROTEIN AND OTHER PROTEINS
WENMAN, WM
CHAI, J
LOUIE, TJ
GOUDREAU, C
LIOR, H
NEWELL, DG
PEARSON, AD
TAYLOR, DE
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1985, 21 (01) : 108 - 112
[43] YEAST RNA POLYMERASE-II GENES - ISOLATION WITH ANTIBODY PROBES
YOUNG, RA
DAVIS, RW
[J]. SCIENCE, 1983, 222 (4625) : 778 - 782
[44] EFFICIENT ISOLATION OF GENES BY USING ANTIBODY PROBES
YOUNG, RA
DAVIS, RW
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (05): : 1194 - 1198
[45] DISSECTION OF MYCOBACTERIUM-TUBERCULOSIS ANTIGENS USING RECOMBINANT DNA
YOUNG, RA
BLOOM, BR
GROSSKINSKY, CM
IVANYI, J
THOMAS, D
DAVIS, RW
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (09) : 2583 - 2587

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