OZONE-INDUCED METABOLIC CHANGES IN SCOTS PINE - MESSENGER-RNA ISOLATION AND ANALYSIS OF IN-VITRO TRANSLATED PROTEINS

被引：12

作者：

GROSSKOPF, E ^{[1
]}

WEGENERSTRAKE, A ^{[1
]}

SANDERMANN, H ^{[1
]}

ERNST, D ^{[1
]}

机构：

[1] GSF FORSCHUNGSZENTRUM UNWELT & GESUNDHEIT GMBH, INST BIOCHEM PFLANZENPATHOL, D-85758 OBERSCHLEISSHEIM, GERMANY

来源：

CANADIAN JOURNAL OF FOREST RESEARCH | 1994年 / 24卷 / 10期

关键词：

D O I：

10.1139/x94-260

中图分类号：

S7 [林业];

学科分类号：

0829 ; 0907 ;

摘要：

A rapid isolation procedure for intact poly(A)(+) RNA from Scots pine (Pinus sylvestris L.) is described. Translation products of poly(A)(+) RNA isolated from 6-week-old Scots pine seedlings treated with ozone (0.3 mu L/L) for 8 h were analyzed using two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). Both qualitative and quantitative differences in the translation products were observed after ozone fumigation. Expression of specific mRNAs, coding for stress-induced polypeptides between 12 kD and 50 kD, was demonstrated after 8 h of ozone treatment.

引用

页码：2030 / 2033

页数：4

共 30 条

[1] SEASONAL-CHANGES IN THE PIGMENTS, CARBOHYDRATES AND GROWTH OF RED SPRUCE AS AFFECTED BY OZONE [J].