RAT-LIVER-TYPE PHOSPHOFRUCTOKINASE MESSENGER-RNA - STRUCTURE, TISSUE DISTRIBUTION AND REGULATION

被引：34

作者：

HOTTA, K

NAKAJIMA, H ^{[1
]}

YAMASAKI, T

HAMAGUCHI, T

KUWAJIMA, M

NOGUCHI, T

TANAKA, T

KONO, N

TARUI, S

机构：

[1] OSAKA UNIV,SCH MED,DEPT INTERNAL MED 2,1-1-50 FUKUSHIMA,FUKUSHIMA KU,OSAKA 553,JAPAN

[2] OSAKA UNIV,SCH MED,DEPT NUTR & PHYSIOL CHEM,OSAKA 553,JAPAN

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1991年 / 202卷 / 02期

关键词：

D O I：

10.1111/j.1432-1033.1991.tb16375.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have cloned a full-length cDNA for rat-liver-type phosphofructokinase. The similarities of the rat liver-type phosphofructokinase mRNA to the human and mouse counterparts were 94% and 99% in their amino acid sequences and 88% and 94% in the nucleotide sequences of their coding regions, respectively. Rat liver-type phosphofructokinase mRNA was expressed in all tissues examined, but its level was regulated tissue-specifically. The nutritional and hormonal regulations of the mRNA in the liver were examined in comparison with those of two other key glycolytic enzymes, glucokinase and L-type pyruvate kinase. The level of liver-type phosphofructokinase mRNA was essentially unchanged by starvation (72 h) or diabetes. The mRNA level also did not change significantly on refeeding starved rats on a high carbohydrate diet, or treating diabetic ones with insulin. These results suggested that rat liver-type phosphofructokinase mRNA in the liver was not under control of diet or insulin, in contrast to glucokinase and L-type pyruvate kinase.

引用

页码：293 / 298

页数：6

共 41 条

[11] MULTIMOLECULAR FORMS OF PYRUVATE-KINASE FROM RAT AND OTHER MAMMALIAN TISSUES .1. ELECTROPHORETIC STUDIES [J].