RAPID ISOCRATIC REVERSED-PHASE HPLC OF TAXANES ON NEW COLUMNS DEVELOPED SPECIFICALLY FOR TAXOL ANALYSIS

We describe a new HPLC method for detection of taxanes from crude extracts, using new specialty columns developed for HPLC analysis. Taxol [1] and cephalomannine [2] were separated in 13 minutes using an isocratic elution. Additionally, there is baseline separation between cephalomannine [2], 7-epi-10-deacetyl-taxol [3], and taxol [1]. Elution of 7-epi-taxol [4] occurs in 20 minutes. We compared chromatography of taxanes on specialty, C18, and phenyl columns. This isocratic HPLC method permits rapid analysis of large numbers of crude extracts, with excellent resolution of taxanes, enabling the detection of 7-epitaxol [4] in 20 minutes.