BIOCHEMICAL-PROPERTIES OF ENDOTHELIN CONVERTING ENZYME IN RENAL EPITHELIAL-CELL LINES

被引：21

作者：

TAKADA, J ^{[1
]}

HATA, M ^{[1
]}

OKADA, K ^{[1
]}

MATSUYAMA, K ^{[1
]}

YANO, M ^{[1
]}

机构：

[1] BANYU PHARMACEUT CO LTD,CENT RES LABS,2-9-3 SHIMOMEGURO,MEGURO KU,TOKYO 153,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 182卷 / 03期

关键词：

D O I：

10.1016/0006-291X(92)91886-U

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This is the first report clearly demonstrating the presence of endothelin (ET) converting enzyme (ECE) in non-vascular cells (renal epithelial cell lines, MDCK and LLC-PK1). ECEs derived from these epithelial cells were very similar to the endothelial ECE in the following biochemical properties: 1) The optimum pH was 7.0; 2) the Km value for big ET-1 was approximately 30 μM; 3) the enzyme was potently inhibited by EDTA, o-phenanthroline and phosphoramidon; and 4) the enzyme did not convert big ET-2 or big ET-3. These data suggest that phosphoramidon-sensitive ECE is involved in the processing of big ET-1 to ET-1 in the renal tubule. © 1992.

引用

页码：1383 / 1388

页数：6

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