ENHANCEMENT OF SUPEROXIDE PRODUCTION OF POLYMORPHONUCLEAR NEUTROPHILS BY OFLOXACIN AND THE EFFECTS OF THE INHIBITORS OF PROTEIN-KINASE-C

被引：10

作者：

NAGAFUJI, T ^{[1
]}

MATSUMOTO, T ^{[1
]}

TAKAHASHI, K ^{[1
]}

KUBO, S ^{[1
]}

HARAOKA, M ^{[1
]}

TANAKA, M ^{[1
]}

OGATA, N ^{[1
]}

KUMAZAWA, J ^{[1
]}

机构：

[1] KYUSHU UNIV,FAC MED,DEPT UROL,3-1-1 MAIDASHI,HIGASHI KU,FUKUOKA 812,JAPAN

来源：

CHEMOTHERAPY | 1993年 / 39卷 / 01期

关键词：

PROTEIN KINASE-C; STAUROSPORINE; H-7; CHEMILUMINESCENCE; OFLOXACIN;

D O I：

10.1159/000238977

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Polymorphonuclear neutrophils (PMN) represent an important defense mechanism against bacterial infection. Superoxide is one of the most important factors released by PMN following various stimulations including bacteria. Augmentation of chemiluminescence response of PMN stimulated by phorbol myristate acetate was observed following the addition of 25-200 mug/ml of ofloxacin, a new quinolone antimicrobial agent. In addition, the effects of two inhibitors of protein kinase C, staurosporine and H-7, were examined. The augmented superoxide production was inhibited by 1 or 2 muM of staurosporine or 50 or 100 muM of H-7. These results suggest that ofloxacin augments superoxide production of PMN and that this augmentation is probably due to the enhancement of leukocyte protein kinase C.

引用

页码：70 / 76

页数：7

共 12 条

[1]

ELIOPOULOS GM, 1989, QUINOLONE ANTIMICROB, P35

[2] CHARACTERIZATION OF RECEPTOR TYROSINE-SPECIFIC PROTEIN-KINASES BY THE USE OF INHIBITORS - STAUROSPORINE IS A 100-TIMES MORE POTENT INHIBITOR OF INSULIN-RECEPTOR THAN IGF-I RECEPTOR [J].

FUJITAYAMAGUCHI, Y ;

KATHURIA, S .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1988, 157 (03) :955-962

[3]

GEISSLER JF, 1990, J BIOL CHEM, V265, P22255

[4]

GERORD I, 1986, J CLIN INVEST, V77, P61

[5] DIFFERENTIATIVE ACTION OF K252A ON PROTEIN KINASE-C AND PROTEIN-A CALCIUM-UNRESPONSIVE, PHORBOL ESTER PHOSPHOLIPID-ACTIVATED PROTEIN-KINASE [J].

GSCHWENDT, M ;

LEIBERSPERGER, H ;

MARKS, F .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1989, 164 (03) :974-982

[6] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY MEASUREMENT OF ANTIMICROBIAL CONCENTRATIONS IN POLYMORPHONUCLEAR LEUKOCYTES [J].

KOGA, H .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1987, 31 (12) :1904-1908

[7]

MATSUMOTO T, 1991, EUR UROL, V20, P232

[8]

MATSUMOTO T, 1991, INT J EXP CLIN CHEMO, V4, P125

[9] A SENSITIVE AND SPECIFIC CHEMI-LUMINESCENCE METHOD FOR ESTIMATING THE ABILITY OF HUMAN-GRANULOCYTES AND MONOCYTES TO GENERATE O2- [J].

NISHIDA, A ;

KIMURA, H ;

NAKANO, M ;

GOTO, T .

CLINICA CHIMICA ACTA, 1989, 179 (02) :177-182

[10] STAUROSPORINE, K-252 AND UCN-01 - POTENT BUT NONSPECIFIC INHIBITORS OF PROTEIN-KINASES [J].

RUEGG, UT ;

BURGESS, GM .

TRENDS IN PHARMACOLOGICAL SCIENCES, 1989, 10 (06) :218-220

← 1 2 →