THE DROSOPHILA CATION CHANNEL TRPL EXPRESSED IN INSECT SF9 CELLS IS STIMULATED BY AGONISTS OF G-PROTEIN-COUPLED RECEPTORS

被引：77

作者：

HARTENECK, C ^{[1
]}

OBUKHOV, AG ^{[1
]}

ZOBEL, A ^{[1
]}

KALKBRENNER, F ^{[1
]}

SCHULTZ, G ^{[1
]}

机构：

[1] FREE UNIV BERLIN,INST PHARMAKOL,D-14195 BERLIN,GERMANY

来源：

FEBS LETTERS | 1995年 / 358卷 / 03期

关键词：

NONSELECTIVE CATION CHANNEL; G-PROTEIN; HORMONAL EFFECTS; TRPL; DROSOPHILA ION CHANNEL; BACULOVIRUS SF9 SYSTEM;

D O I：

10.1016/0014-5793(94)01455-A

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Structures and regulations of vertebrate channels responsible for sustained calcium elevations after hormone stimulation are largely unknown. Therefore, the Drosophila photoreceptor channels, trp and trpl, which are assumed to be involved in calcium influx, serve as model system. trpl expressed in Sf9 cells showed spontanous activity. Hormonal stimulations of calcium influx (detected by fura-2) and of an outwardly rectifying current were observed in Sf9 cells coinfected with baculoviruses encoding trpl and various heptahelical receptors for histamine, thrombin, and thromboxane A(2), all known to cause phospholipase C-beta activation in mammalian cells. Although the identity of the G-proteins and of possible second messengers involved need to be clarified, it is clear that trpl represents a receptor/G-protein regulated cation channel.

引用

页码：297 / 300

页数：4