NORMAL-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF NEOMYCIN SULFATE DERIVATIZED WITH 1-FLUORO-2,4-DINITROBENZENE

A high-performance liquid chromatographic (HPLC) assay method has been developed for quantifying neomycin sulfate powders and oitments containing neomycin. A dinitrobenzene derivative of neomycin is formed and then chromatographed isocratically on a normal-phase system. Neomycin B and C can be separated and quantified with a relative standard deviation of approximately 1%. When monitored at 350 nm,the method is sensitive to about 1 ng of neomycin base per column injects. Gradient elution and isocratic HPLC methods for the analysis of derivatized neamine have also been developed. The relative standard deviation is less than 1% for the isocratic method. The HPLC method is applicable to the analysis of other aminoglucoside antibiotics. © 1979.