ODOR-INDUCED PHOSPHORYLATION OF OLFACTORY CILIA PROTEINS

被引：53

作者：

BOEKHOFF, I

SCHLEICHER, S

STROTMANN, J

BREER, H

机构：

[1] University Stuttgart-Hohenheim, Institute of Zoophysiology

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1992年 / 89卷 / 24期

关键词：

2ND MESSENGER; PROTEIN KINASES; PROTEIN KINASE INHIBITORS; PROTEIN PHOSPHATASE;

D O I：

10.1073/pnas.89.24.11983

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Stimulation of isolated rat olfactory cilia in the presence of [gamma-P-32]ATP leads to a significantly enhanced incorporation of [P-32]phosphate. Depending on the type of odorants applied, the induced phosphorylation is completely blocked by specific inhibitors of either protein kinase A or protein kinase C. Time-course experiments indicate that the odor-induced modification of ciliary proteins is transient; the intensity of labeling decayed over time (1-10 sec). Separation of ciliary proteins by SDS/polyacrylamide gel electrophoresis followed by autoradiography demonstrated that upon stimulation with lilial, a single polypeptide (50,000 Da) was phosphorylated; the size of the modified protein is in line with the hypothesis that odorant receptors are phosphorylated subsequent to activation by specific odors.

引用

页码：11983 / 11987

页数：5