A BLOCKING ELISA USING A MONOCLONAL-ANTIBODY FOR THE SEROLOGICAL DETECTION OF MYCOPLASMA-HYOPNEUMONIAE

被引：22

作者：

LEPOTIER, MF ^{[1
]}

ABIVEN, P ^{[1
]}

KOBISCH, M ^{[1
]}

CREVAT, D ^{[1
]}

DESMETTRE, P ^{[1
]}

机构：

[1] RHONE MERIEUX,IFFA LAB,F-69342 LYON 07,FRANCE

来源：

RESEARCH IN VETERINARY SCIENCE | 1994年 / 56卷 / 03期

关键词：

D O I：

10.1016/0034-5288(94)90151-1

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

A blocking ELISA was developed by using a monoclonal antibody (4082-05-344-18) which specifically detected an epitope on the Mycoplasma hyopneumoniae 40 kDa membrane protein without cross-reacting with M flocculare or M hyorhinis. The results obtained with sera from specific pathogen-free pigs inoculated with M flocculare or M hyorhinis confirmed the specificity of the assay. An immunoblotting procedure was used to characterise the antibody response of pigs experimentally infected with M hyopneumoniae. Antibodies to the 40 kDa antigen were detected two weeks after infection and remained as major markers for at least 20 weeks. Cross-reacting antibodies to this antigen were not detected in convalescent sera from piglets infected with M flocculare ou M hyorhinis. Sera from experimentally infected pigs were compared by means of the blocking ELISA and an indirect ELISA. The kinetics of ELISA antibodies after experimental inoculation were also studied. The detection of antibody was rather more stable for a longer time with the blocking ELISA than with the indirect ELISA. In an evaluation of more than 1000 sera from the field there was excellent agreement between the two methods.

引用

页码：338 / 345

页数：8

共 28 条

[1] SPECIES-SPECIFIC ANTIGENS OF MYCOPLASMA-HYOPNEUMONIAE AND CROSS-REACTIONS WITH OTHER PORCINE MYCOPLASMAS [J].