CARNOSINE-L-H-3 BINDING IN THE OLFACTORY-BULB .2. BIOCHEMICAL AND BIOLOGICAL STUDIES

被引:16
作者
HIRSCH, JD [1 ]
MARGOLIS, FL [1 ]
机构
[1] ROCHE INST MOLEC BIOL,DEPT PHYSIOL CHEM & PHARMACOL,NUTLEY,NJ 07110
关键词
D O I
10.1016/0006-8993(79)90805-9
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
During the first 10 days after peripheral deafferentation of the mouse olfactory bulb stereoselective binding of l-[3H]carnosine declines markedly. The initial phase of this decline is due to a decrease in binding site stereoselectivity, which is then followed by a loss of assayable binding sites. The specificity of inhibition of l-[3H]carnosine binding by various peptides is also altered after denervation. Competitive inhibitors of carnosine binding become less potent after denervation, while analogues which are not competitive inhibitors remain equipotent before and after denervation. Several carnosine analogues that are normally poor inhibitors become more potent after denervation. Treatment of bulb membranes with trypsin, RNase and hyaluronidase, but not DNase or collagenase, resulted in significant alterations in carnosine binding. l-, but not d-carnosine, protected the binding site from trypsin digestion, and induced additional binding in bulb membranes in a dose-and temperature-dependent fashion. Preincubation of membranes with l-carnosine also led to the induction of additional carnosine binding in membranes from cerebral cortex, cerebellum and deafferentated bulbs but not from muscle. Bulbs from newborn mice contain about one-half of the adult levels of binding and no significant sex differences in carnosine binding were detected in bulbs from adult rats. l-[3H]carnosine binding was two-fold higher in the anterior compared to the posterior portion of the bulb, but there were no significant differences in binding of opiate, GABA, α-adrenergic, muscarinic cholinergic, benzodiazepine or glutamic acid receptor ligands. © 1979.
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页码:81 / 94
页数:14
相关论文
共 28 条
[1]  
BEAUMONT K, 1977, 7TH SOC NEUR ANN M, V3, P453
[2]  
BENNETT JP, 1976, MOL PHARMACOL, V12, P373
[3]   OLFACTORY, THERMAL, AND TACTILE CONTROLS OF SUCKLING IN PRE-AUDITORY AND PRE-VISUAL RATS [J].
BLASS, EM ;
TEICHER, MH ;
CRAMER, CP ;
BRUNO, JP ;
HALL, WG .
JOURNAL OF COMPARATIVE AND PHYSIOLOGICAL PSYCHOLOGY, 1977, 91 (06) :1248-1260
[4]  
CAGAN RH, 1977, 7TH ANN M SOC NEUR, V3, P77
[5]   SOCIAL OLFACTION - REVIEW OF ROLE OF OLFACTION IN A VARIETY OF ANIMAL BEHAVIORS [J].
CHEAL, ML ;
SPROTT, RL .
PSYCHOLOGICAL REPORTS, 1971, 29 (01) :195-+
[6]   EFFECT OF DIETARY HISTIDINE LEVEL ON CARNOSINE CONCENTRATION OF RAT OLFACTORY BULBS [J].
CHUNGHWANG, E ;
KHURANA, H ;
FISHER, H .
JOURNAL OF NEUROCHEMISTRY, 1976, 26 (06) :1087-1091
[7]  
Cuatrecasas P, 1976, Adv Protein Chem, V30, P251, DOI 10.1016/S0065-3233(08)60481-7
[8]   BINDING OF OPIATES AND ENDOGENOUS OPIOID PEPTIDES TO NEUROLEPTIC RECEPTOR-SITES IN CORPUS STRIATUM [J].
CZLONKOWSKI, A ;
HOLLT, V ;
HERZ, A .
LIFE SCIENCES, 1978, 22 (11) :953-962
[9]   DENERVATION IN PRIMARY OLFACTORY PATHWAY OF MICE .2. EFFECTS ON CARNOSINE AND OTHER AMINE COMPOUNDS [J].
FERRIERO, D ;
MARGOLIS, FL .
BRAIN RESEARCH, 1975, 94 (01) :75-86
[10]  
HARDEN TK, 1977, J PHARMACOL EXP THER, V203, P132