APPLICATION OF SCANNING ELECTROCHEMICAL MICROSCOPY TO BIOLOGICAL SAMPLES

被引：114

作者：

LEE, CM ^{[1
]}

KWAK, JY ^{[1
]}

BARD, AJ ^{[1
]}

机构：

[1] UNIV TEXAS, DEPT CHEM, AUSTIN, TX 78712 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1990年 / 87卷 / 05期

关键词：

Profilometry; Topography; Voltammetry;

D O I：

10.1073/pnas.87.5.1740

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The scanning electrochemical microscope can be used in the feedback mode in two-dimensional scans over biological substrates to obtain topographic information at the micrometer level. In this mode, the effect of distance between a substrate (either conductive or insulating) and a scanning ultramicroelectrode tip on the electrolytic current flowing at the tip is recorded as a function of the tip x-y position. Scans of the upper surface of a grass leaf and the lower surface of a Ligustrum sinensis leaf (which show open stomata structures) immersed in aqueous solution are shown. Scans of the upper surface of an elodea leaf in the dark and under irradiation, where the tip reaction is the reduction of oxygen produced by photosynthesis, demonstrate the possibility of obtaining information about the distribution of reaction sites on the substrate surface.

引用

页码：1740 / 1743

页数：4

共 15 条

[1] PROBING BRAIN CHEMISTRY WITH ELECTROANALYTICAL TECHNIQUES [J].